XB-CLONE-3552664
Clone Name: IMAGE:7714617
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Gene:
dgcr8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX973736
[+]
caagaaagcaatttgggatgacagaaacatttgtcagattaacctcagacgcttttctatagtcagtgtttcagatattattttatctgttttattattt
gaagtaaaagtattggctttttttttgttaaggaagtagaatcaacattaagtgcgccgtaccagaaccagaacatggggcgcgactttgctttgtctac
ttggcgtgtttcagagagaaaatgtgaacaagtcatgtgtgtgttgctattgctataggcccatttggttcgactagcatatgcaagctgtattcctatt
ggtctcccattcccaggagtgtttttaggtcttaatgtttaaatgcatgtaaaagcattggatgttttatgcttcattgttaatggatggtacagtagct
tacaggcaccctttcctgcatggaatcctacattagaaatttcactttattattgggttgtggtttttgatgccagaatatgaaacgttccacattatag
agtattatggtgtctgatgtggtgagtttgggtggtgttgctgttgcatttctgctttgttccctggcagctcagttactgccggcaggtaatgcgcaac
tgaatgtatctccagcgtcaccatttgggctctgccacattcattcctacccatgcgtctgacagcgggcgtttctagggaatcgcctgccagtctgttg
ctattctttgcatgaatgcaggtgagttggtttaacactgctnctattatatatgtctg
3' EST: CX973735
[+]aaatttttattgcttatactaatgccgaacaaaatatttccaacagtactgaagatacaatgttcgagtcggtgagagcggcaatctagttgagcggggt
acagattgaacaactcagtcacaagaatcagacacaattcatacaacaaccgttacgttaggcacaattagctgaagtcccacatgattacacacaaggc
ttaagctgctcactataaatgtacagcaaatgatttcatcaacatgaacagtccagcccagaacatcaaatccatccaaatgtccacaatgacagttact
tctatttcagtcgtcaatagcaaacagtagtgctatataaagcgattacagggagacatcttaatgcatgaaatggcagaattccgttttctagttaaag
gccgattaaatataaatacaaatattaaagagccattaagacatcaggcagcatcataagtaagtggctctcaaaccaaagtgtagaaaccctggccagg
aactacaagttaattacatcagacatatataataagagcagtgttaaaccaactcacctgcattcatgcaaagaatagcaacagactggcaggcgattcc
ctagaaacgcccgctgtcagacgcatgggtaggaatgaatgtggcagagcccaaatggtgacgctggagatacattcagttgcgcattacctgccggcag
taactgagctgccagggaacaaagcagaaatgcaacagcaacaccacccaaactcaccacatcagacaccataatactctataatgtggaacgtttcata
ttctggcatcaaaaaccacaaccccataataaagtgaaatttctaatgtaggattccatgcaggaaagggtgcctgtaagctactgtacca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: