XB-CLONE-3552605
Clone Name: IMAGE:7714527
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Gene:
cyp4v2.2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX973775 [+]
cgctgctgcacatcccgccacttgcctgtgtttcactgcaagacatagccatggagctggggggagaagtccatctgttggtgtgggtggcggcggccgt
ggtgctgctgaccctgctggccctctccattctgcctgccctgcaggactacgtgcgcaaacgcagaattttgaaaccgatcccgggccccggtcccaat
tacccactcattggcgacgcgctgttcctgaagaataacggaggagatttctttctccagatctgcgaatacaccgagagctatagattgcaaccacttc
taaaggtttggattggcacaattccctttatagtagtctaccatgcagatactgtagagttctgagctcatctaaacatatggacaaggctttcctctat
aaatttcttcacccttggcttggtaaaggactgctaacaagtacaggggaaaagtggcgttcaagaagaaagatgataactccaacctttcattttgcaa
ttctctctgagtttttggaagtcatgaatgaacaatccaagatattagtggagaagctccagacgcatgttgatggggagtcttttgattgctttatgga
tgtgacactatgtgcattggacattatatcagaaacagccatggggaggaaaatacaagcacaaagcaatagagattctgaatatgttcaagctatttac
aagatgagtgatattattcagcgcagacagaaaatgccatggttatggcttgactttttgtatgcacacttgagagatggaaaggagcatgataaaaacc
taaaaatcctccactcctttactgataaggctattctagaaagagc
3' EST: CX973774 [+]gaaaaaagcaacaaggttaaagcattgcaaggaaagtgcatatttattagccaaaatgtcacatcagtctattgattgtccctttgccatacatctttgt
acactggacatgttaccctgcacattataagaatataacaatataagtatttaacaatatatgtatctaatataagtattaatatacgtatttaatatta
aaaaaaagtatttaacaacatttgttaaatacagaaagagtgccttcatagagggcatgaaacacaggatttccaatatacagtatatcatacacttaaa
agaaggattacaccaggggtgctcagactttgctgaggtgggatatattgggttatactacagtatgtgaatgagttgtaagtgcaaaacagatgcacac
agatttttgcagagtaccccaccataccttacatccaataggtggtgatcatatcaagtacaaagaattcctatccaagtacaaattaggtctcgataaa
ctctgttctttctgtaaaactggcatctgatggcaccattggtaaaaatagaagttacatgtatcaaatgcaatgtctgtaatagggtaatacaaacaga
gcaaaaccaaaactctgaataaatcaatcatagcttctgaaagtaaagcagaattacaaggtactttctttccatacagttgctgctatttagaattaga
ttgcaatgttatatttttaaagtatcttttacattctgtctagtaaccattcaaaattttgtgatatactgtgatatatgtataaacatagaacanaatg
accagcaattataattaataaccagtattagttgccaccccagaattagaaattaaatgcttatgtgtttcaggcagtaggggcagtttctctgttc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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