XB-CLONE-3552581
Clone Name: IMAGE:7714529
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Gene:
cxcl14
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX973791 [+]
caactttctacgtaaggaaaatacataaatattacatgataatttagcaatacaatacattacatgtatagataatatacaaatatttatatgaagcgtt
tctgttaaattttatttacaaattatagttattattgaatgatctgctttctgtgggaacttaagactgacacgtgtgtgtgttaccgtaaaagcttgac
gttctatagcaatgaattatgttcatttgcaaatctaaaatgggttgcacatctctgacagtttacattattattctattatttgtatatatgcacacat
acacatatatttaccccaaaaagtcccattaagttgacattcaaaaactttgtggcatgtccactgtccttaggatattttagataatgtaccattataa
tagggttatgtaacaaacggtacaaagtctgcccaagagcattaacccatagtaactaatcagcagaaagcattacctgtataaaagcaaacatcttatg
gttgctatgggttactgctgctgggcaaacctagctcctttttttgacacatgagggtaaatatcctaagcaggacgtataaaggcattttgtgaagttg
gctacgaacaccggcagccaaaatgtccaatataggacctcatctgacaatttgctaaatgattaaatttagaagatagctcagtattcatttgtccttt
gattttaaatacgtgccactgacttcctgctgaatggatttactaatacgtcacccagaatctggtcaattccatgtagttgaccagagtatttgggtct
tttggagtaagtaaacacagttgtgcagttttgt
3' EST: CX973790 [+]ttctggcatttgggtcaatttaggttcctggtttggatactgggactgagaaatataggaaattacactttagcacatgactatttagttactattgatt
actgtatgtgtcggttactaatttacgtatgggacttaatctggatacacatgacaggtactgatggcaaggttttaacaaaactgcacaactgtgttta
cttaactccaaaagacccaaatactctggtcaactacatggaattgaccagattctgggtgacgtattagtaaatccattcagcaggaagtcagtggcac
gtatttaaaatcaaaggacaaatgaatactgagctatcttctaaatttaatcatttagcaaattgtcagatgaggtcctatattggacattttggctgcc
ggtgttcgtagccaacttcacaaaatgcctttatacgtcctgcttaggatatttaccctcatgtgtcaaaaaaaggagctaggtttgcccagcagcagta
acccatagcaaccataagatgtttgcttttatacaggtaatgctttctgctgattagttactatgggttaatgctcttgggcagactttgtaccgtttgt
tacataaccctattataatggtacattatctaaaatatcctaaggacagtggacatgccacaaagtttttgaatgtcaacttaatgggactttttggggt
aaatatatgtgtatgtgtgcatatatacaaataatagaataataatgtaaactgtcagagatgtgcaacccattttagatttgcaaatgaacataattca
ttgctatagaacgtcaagcttttacggtaacacacacacgtgtcagtcttaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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