XB-CLONE-3552438
Clone Name: IMAGE:7714590
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Gene:
prkd3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX973885 [+]
caggaaaacacttttctgaaaaatattgaaatatttcaattatatacatgaatacacagataaataactactactcctaccctaaagtagttccaactta
ctatcagataatcaaatccagaaagaacagcctctttgaaatatacaataaaaaaacatacattttatagcatgacataaacaattttcatgtaacagca
aatctgattattctccactgcagggatagtattactcgtcctgctgagaaaacaatggagctgctattctttagaggtacaacatttaaaaggcttggca
gactgtattgtagctttgtcatatgtactgatagcattgtcacatgcagtgcctcttcttgccatttttaggggaaggaaaatcatttccatagcccctg
acatataaaaaaaaactgaaatatttgaataaaactcctttcaaaactgacagaaccttaatttagatcaacaagattaatcagaggaatattttgtttc
cagaaaaacagtaggtatctttataaaaaggtcctgcattattagccacatacaagcttgaaaggattcaaatctcaccactttcactaatgcaaacact
cgggggccatactcgtttcattctaatcagcttaaaatttcccaagcacataaaaacattttcattcaatttagacttggacctaataaatataaaatgt
tgtaaatctggtctctttattcctgaatgtcgtcctcttcagtttcatcatattgtggccccatgacaaagtgttgcgggtacaaaatatcacattcctt
agcgtatttctgccaacgggcatcatcactctcgtgggtaatgtacctctgcccaactctgggttctagttcc
3' EST: CX973884 [+]ttcggcacaatttgagctcacaaaatgttcttgaacggctttttcccaacaattttatccaggaaggggacttgattgaagtggtgttgcctgcatctgt
gtccctggatttctcaatacgtcctcacattcttttagttcattcctacaagtctccagccttttgtgactactgtggagaaatgttgtggggactggta
agacaggggttaaaatgtcaaggatgtggtctgaattatcataagagatgtgcctttaagatacccaacaattgcaggggtgttaaaagaggtcaacttt
ccagtcgctctttcactggaaaccaaacaaacaaacctaaagcatcacccataccatcaggcgtcagtctggaggacctgggggcacattactcagattc
cagggatatttaccagcttattcaaaagaagccccgggtaccttccatcatcggacgccctatatggaaggactgcgaggaaataaacaaagttaaagtc
cctcatactttccaggtccattcctatacccggcctacaatctgtcagcactgcaggcgcttattaaagggcctcttcagacagggcatgcagtgtaaag
attgtcgttttaactgtcaccgacgatgtgtggaatatgtccctgatgagtgcctaggtgaaatatcccatcctgatgaagaagatgcagaggaagatat
tgatagtgtgagacaaacaaaccttggtacaaatcatcaggatgaagatgaaatgtcattcaaggngtctgaatcatcagatctttcatgcaatattgaa
aactcaatgtttgacagtaacttcattccactcatgagagttgtgcagtctgtgcgacataataaaaagagaatagntatgtgttaatggaagctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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