XB-CLONE-3552435
Clone Name: IMAGE:7714638
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Gene:
enpp7
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX973887 [+]
cagtatcttacaaatgttacacagcattaatctacagatatccccatttgttttattccggtttttaatggccatccgccagctggaggtctagtgtgag
ggatcagtggatgtcatttctgttgagtgctaactcttggtgcatgctggaacattacattgatcatagagtaaagccaactgaaaagggaatattggct
taggatggaagaaagtccttacagcctctaagagttaggatatgtctttttaggatgtcaacttttatctgtaatggtctccgtactcaaagatttctgg
gctcttttcctctttttgtgtctcttgacagaaacagtaatcattgtaataataaagctgagaagaagcacccccagaacagctgacagtccaattgttg
catgtagaatggatgctgggaaacatgctgggttgggatctttgtccaccagcatgttccgtgtcacatctagggatccatcattgggctcaggtttgac
acccagcagctcacacatcagggcatacacatgaacgctctcaaaaggctccacaaccaaatcctgcttaaaggatggacccacagcacggaatatggtc
ttcatttccatatcatcattgtcaaagccatgctcccctttattaaactgtaactttgcaaacccatgaacgacatatcctggatctccatatacaacaa
gcggtgtaatccgagcattgttggaatagtgcagcctggctggtacttcttcttttttaaaaacattgagcttagggtgagcattctttagggcattata
taccttttctaaaata
3' EST: CX973886 [+]cacaaggctcctcacaaactcttgcttatctcctttgatggctttcgttgggattatgatcaagatgttgatacccctaatatgaacataatggccagag
acggtgtgaaagccaagtacatgacccctgcttttatcactattaccagtccatgtcactttacactacttacaggaaaatacatagaaaaccatggagt
tatccacaacatgtattttgatgtcaccactggtcagaagataccataccacggcacacagggcgtgtccatgtggtgggataatggtagccttcctatt
tggatcacagctcagagacagggccttaacactggttcgcttttctttccgggtgggaatgcaacatatcgtgatgagaatgtgaatgtaaaaaaagtag
agtcacgcacacacatatggggcaatgaaacagagtggagagaaaatgttgacactgtgatgcgttggttaacagagcaggatttggattttgtagcttt
gtattttggtgaaccagatagcactgggcacaagtatgggccagactcagaagagcgcaagcagatggtcagccaagtagaccgaaccgtcggatatata
agaaagcgtgtgaaagatcatggtctagaggatagattaaacatcatcatcacggcagatcacggcatgcgaacagtacacaagggccctgatgagatta
tcctcaataacctaccanacttctcactttctgaccttcagtttcatttggtagattatggacctagtgngcttttgatacctaangaaggtaatttaga
aaaggtatataatgccct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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