XB-CLONE-3552340
Clone Name: IMAGE:7714456
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Gene:
gcdh
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX973953 [+]
aaataattaaactaaattgaacaaacaacagatttttgactccgctgtacaggaataggcattgcttccgacagcccaggttataacagaatgagtcagc
aaagcctgaaaaactactgggcaccaatttcatattttaaaaccccaattacctctatttgactccccctgctctaccttaagagtgacttcaggcttgg
atactgcatatatctatcccccagcctatagtttttacaccagacttaaccacccctgaaacagatgtgggggaaaccctgtcatcaacgagccaatgcg
gtccccgatgcgactagattttctaacctgcccgattgagatctggccgatttcagaccagatatcggtcgggcaggcccattggcagtgcccatacacg
ggccgattagctgctgaattggtctaaggggccaatatcggcagctagaatcggcccgtgtatggggacctttagcctggttcaagtctgtcattcaaac
cactgcctcaatgctatgtttattgggaccgtagagaccaattgcaaactgcagaacaaaaaaaaaaaagaactgcaaactgttctaaaggcgaacaacc
cctttaatggaaactgaatgttttctctctacagtggaatcgtgagcgatggcagaatgctgtacagttgctgcagacggtgggaggaatctggaaccga
gtggagcggaatcacttgcctacagtaaatgcctgcagccctgtgatggctctgccaaggatcagtgcatgcacatcgtgggtaccctcgtaagtgttca
ccgattccagattcatgacgtgcctgatgatgtggtactcgtctgaaatcccatttcccccc
3' EST: CX973952 [+]cccgagtccagttcgactaccaggatgccctcagcctggactcccagctgacagaagatgagattatgatacgggacagtttccgcgcttactgccagga
gaagcttatgccacggatccttatggccaatcgcaaggaagtatttgaacgggacatcatctccgagatgggagagctcggtgttctgggatctacaatt
aaaggatatggctgtgctgggacatcgtatgtggcctatgggctgttggcacgagaggtagagcgagtggacagcagctatcggtcagttatgagtgtcc
aatcgtctcttgttatgcatcctatcgctgcatatgggagtgaagaacagaagcagaaatacctgcccaggctggccagcggagagtggttggggtgttt
cggtctcacagaaccaaaccatggaagtgacccagctggaatggagaccagagccagatacaatccctccagcaagacctacacgctgaatggcgccaaa
acctggatcaccaattccccaatagcagacctgtgtgtggtgtgggcgatatgcgaggacggcaaggtcagggggttcctgatagagagaggaacgaagg
gactgtctacccctaaaatagagggcaagttctctctcagagcctcagccactggcatgatcctgttggaagacgtggagattccagaggaaaacctcct
ccctaatgtttctgggctggggggccccttcggttgtctcaataatgctcgctatggcatttcctggggggcgctgggtgctgcagaattttgctttcac
acagccagacagtacaccttggataggatccagtttggcgttcctcttgccaggaaccagcttatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: