XB-CLONE-3552285
Clone Name: IMAGE:7714652
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Gene:
cfap97
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX973991 [+]
ggtaccttagcgcaactgcattgtcgcccagacccggaaaagcatcagtaagccgtctcagcccctctgtatcttcaggaggcttttcaaggatgtccag
tgcaaccaatcgaagcgaaaggacaggatccagtggggctctgctgaggcctacaagatcgggcaatgtccgagcagcttggcagtgaatatcttaaaat
atgaagttttgtatttcagtgtcattccagtgtctatgctgctattgtgaactactttgtgttgaataaagttgttgtgttgggagaatattaatatgat
acatgcatatacatgctttttatagatatatattttttgatataaagtaaataatttgctattgtcagtaaaatgtaaccgctttgaaagtgaatgcctc
taattcaaggagaagagtcctgggcttttaaataaaatgcagttgaagtgcaatggtggagcaagacttagcaagggagtgagtatgaaatgaattatgc
agatgtggcactgaggtttgtatggtataaacgtccctttttgaaccagctatttgtttgtcctgtaattgaattcattttatcctgaagttgtgtagcc
tacaagttgttataacagtactgtcattgtggaaagttcagggttcatgagcactatacacatgaatgggattgaaatcccagtgtggctgggccataat
cacatccttggaggcaatttgatttgcagcatgtgtaatggaagacatgtctggctctgatttaagcaatgtaagaagttgcacctgcattaatttgtgc
atacactagtttctctcaataacagaggttttcggggcttgaaaaaactctgggtatatacctgtctaaattgcagtatgtaa
3' EST: CX973990 [+]cagaccaaattattttattttgctccacaatacaaaaagcaaatattaaataacaaaatttagaattatcaaacaggcaaacataacctatatacttttt
tggccaagatcacactatacagcaaaaccactggaagaacaataacaggatacagctgatacctgtgtaatgcaagagaataagtaaattaatgtctgga
ttgtagcaatgcttcatcactgcagatgagttattgtgtagcaacccagactgagtaaataaaagtacaacagtagccccacttgagttacatgaccaaa
aagcaaggggattagaacacaaggatacaccccagctcggagggccacgctcgtgcaatctgaactaccataaatatgaagagatttgtcagtatttaga
actgtgcatttctggatgctttcaatgtcaaactaaaagtgcagttgcgattctgaatgtacagtgctagcaaaagtatttagacccacaaagaaaatct
ttgtttctcggagatatttgtatttcaaagtattaaaacttaaaattagtatttgaaattattagatgttagaaaataaaagcctttaaaagtgaaatat
agcactattattcaatccacattgtataatatcggtagatcatccttttgcagcaataacaagttaaaatctattaggaatgttacagatttgcacagtc
tggaagtgataatggcacttggcaatttgctgagctaaatgatatttgtacactttcaacaatttccaactgtgccacagattttcagttgggctgagat
cagggctttaactgggccactgtagaacag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: