XB-CLONE-3552175
Clone Name: IMAGE:7714614
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Gene:
hla-dra
,
mhc2-daa
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX974065 [+]
cataaggcagaaggcacaaaactaaagaaaatgtcctacgtgagatttaaacagcactctcttataagatataaatctcttaagatatagttctgtcttc
aatcatgtgttgcattccagctaccgcagggttacaagcattattactatactgatcatctctacagaaagacaaaagttaaatgaattatagggttggg
gttacactttttaatagctttatttttgtttaggagatcaagaaaaacagcagggcatccatttagtttgtacagaaagtagcaatcaagatcagggcta
tataagaaaatacaagaaagtacatgactggatagaatactttgcagttctggagctaccagaccatagagcaagccaattaacagaggatgttacaact
tcaacattcaataacgatcctccttgtagctgggggtaatttgatattcattcaaagccaacacatacattgtattttaaatcatcatcaaataaaattg
tgggttagaatgatatacatcacacagaaaccattgcaaatattgaggctgcaattaaatgtaattttcaatttcagagatgcatctaaaagctaagcta
atttaaaaacaaaaaaatcaaggaatcaatctttcatacactgtagcagagattatagtatgtgtgatgcccaatgatatatgagtgctatttcatggtg
aatatgaacagtttctattctgtttgtgtaagcaaggtatattcagcaaaacaaaggagaacagatacaccacattaaaagaatacaaaattgtcccact
gatttgcataccaccctcaagcactagtataagcacttgtcaggtgacagct
3' EST: CX974064 [+]tttatgaatcatacttcactgaaataatattgtaaatttcccctcccaatgcaatatcattttcttcaaattggaaatccaatgtatttacctgttcatt
taaccttaattatcccatcccaaaccattcttgtacttaattctgtttagagaagggcatactgatagccagcaaagatcatcagcactgggttatctga
aaggcactcacataatgccagaagctgcagcattacccagacatttttccactgacactcagcaatttgactgcagtaggaagtttctggagacttgaat
taaactcaaagaaaagtctctggtctcattctgaaataactgtatctatattctgcatggctggaggcatttttttcttttatttcttaaagggataagt
ttaacaaatgcttattttcagtacaactgtacaataacaatgactatttcagttccttcctactagatcctattaaatcccatagatttaagttaaactt
cctccctctggtgtttgtccactacactataaacagacagtctgaactgttacaatttcctacatttgtgatacatctttctgaagcattggcggaatct
caccaatatatatagtccctgtgtgtgcggctcactgtcttgttttttatatatatctaaaggattatgctcagtagggactgaagttaaaaaatatatc
aactaatcaaattgtagcagtttactaaactgctcacattaataaagaatagaaaaacttcataactcttgctgtactttgaaaacagacaatagcattt
gaaaggtgaaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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