XB-CLONE-3552054
Clone Name: IMAGE:7714530
|
Gene:
hnrnpl
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX974145 [+]
aagactgacgcatctgaggacaacgtggcggcttcagcggaagggtacgatgaccctcacaagaccgcggcctccccggtggtccatgtgaggggactta
ttgatggggttgtggaagcggaccttgcagaggctttgcaggagtttggaacgatcagttatgtcgtggttatgcctaagaaaagacaggctctggttga
atttgaggatattcttggtgcatgcaacgctgtaaactacgcagcagacaaccaaatctatgttgcagggcatcctgcatttgtaaattattcaactagc
caaaagatctcccgtcccacagatacaggcgatgattcccggggggtcaataatgttctgctctttaccattctgaatccaatctattccataactacgg
atgtgctgtataccatctgtaatccttgtggacctgttcagcggatagttattttcaggaaaaatggagtccaggccatggtggaatttgactctatgca
aagtgctcagcgtgccaaagcttctctcaatggggctgacatttactctggatgttgtacactaaagattgaatatgctaagccttctcgactgaatgtc
ttcaaaaatgaccaggatacatgggattacacaaatcctgccctgagtggacaaggtaattcacttggttctgttctctgctttttacacatttcctatc
agttagtctgataaattgcacaagtgtcacttcacgctctgtaatgccatctgcttgtctaatagtcttgtttatatattcaacaaatagccattgtttt
ggcttaaactgctttaaaataacttgngacaaaccttttgcactttcctgaa
3' EST: CX974144 [+]gttctcaatgttttattgatatgtgcaagcacatcatcatagcaaaaaaaaaaaaaaaaataaaataggaagtcacaacaatatttcagaacataaaaac
tagctccacaaggaaccaaactaatactaaacatcaaaatcagggctaggaaatcagacaggtcttcttaggaggcatgctgagcagtggagaagcagag
ttttagagtgtaggggtaaggaccatttggatttttcatctggtagtgattcatgagaccgagagtctccagtgcttcgctcttagaatcccactccagc
agtccggaagagcttcgctcactctttccagaaaagattttgattgaagctggcttcttaacacccagttcatcacacatctctataaagttctcctctg
tcacctcaggaggagcattaaagaaatgaagcacactgctcggctgttggattctgttcttggcagcttgctctggggatgtaaatctgttgttacggga
cccactgaagactttaaaactgcaggagccatcttccaagccataggactgcccaggcacaatagactgttgctttgaaacacagacacttagtttttgc
ccaaacatgaagttgttgtttaagtgtgtaatggcacggtctacagcatatccatcagccatctccaccatagctgctccaggtttactcttcatgaatt
ttaccttctccacattaccatacaggcagaagacattaaagactcgatcacagttcatctttgaggggtctaaaccatataccatcagtactggactgtc
tgcatgtgggccatactctggtggtggtggtggtggcggatgcccatatt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: