XB-CLONE-3551895
Clone Name: IMAGE:7714879
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Gene:
faxdc2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX974250 [+]
tggaactcacagtcttcgcgctggttgaggaaattctcttttattattcacaccggctgggccatcaccctctattgtacaagcgcatccacaagaagca
ccatgaatggacggcacctgtaggagtggtgtgcctgtatgcacatcccctggagcatattttttccaacatgctgccatccatggtcggccctatggta
atgggatcccacgtggccacaaccatgctgtggttctgcctggctttgatcaccaccaccatctcacactgtggctaccacctgcctttcctgccttctc
cagagttccatgattttcaccatctcaagtttaaccagtgctacggagtacttggggtattggatcatctgcatggaacagatctgatgttcaagcaaac
caaggcttatgaaagacacattctattaatgaaccttacaccactaagtcagagcattcctgacacccctaaaaagtcccaatagtctaaagggggaaat
gttagaagagaagttgtccctcttgaacagagacctgcaaggatatggtttgcccttaatgttgtgctaatgtactagcaacatccagcgggtttgggtg
tgttggggcacccactccagtttttgactgacttatgatactaattatctctgtgattaaatgataattagaaggttagaaattgttcatggctgtaggt
gcatagaanaaaaacccagtgtctgtggatatttagctgtagatttagggtccattgagaacactgacatcactgtgcaccatagtggcccattaggtac
ggatgttaccgtatgtacattattcagtagtga
3' EST: CX974249 [+]gcacacgagttagttgtgattagtgtaagaatatgatgctgttgcacccacccaatcccactcgtcactactgaataatgtacatacggtaacatccgta
cctaatgggccactatggtgcacagtgatgtcagtgttctcaatggaccctaaatctacagctaaatatccacagacactgggttttttttctatgcacc
tacagccatgaacaatttctaaccttctaattatcatttaatcacagagataattagtatcataagtcagtcaaaaactggagtgggtgccccaacacac
ccaaacccgctggatgttgctagtacattagcacaacattaagggcaaaccatatccttgcaggtctctgttcaagagggacaacttctcttctaacatt
tccccctttagactattgggactttttaggggtgtcaggaatgctctgacttagtggtgtaaggttcattaatagaatgtgtctttcataagccttggtt
tgcttgaacatcagatctgttccatgcagatgatccaataccccaagtactccgtagcactggttaaacttgagatggtgaaaatcatggaactctggag
aaggcaggaaaggcaggtggtagccacagtgtgagatggtggtggtgatcaaagccaggcagaaccacagcatggttgtggccacgtgggatcccattac
catagggccgaccatggatggcagcatgttggaaaaatatgctcca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: