XB-CLONE-3551828
Clone Name: IMAGE:7714981
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Gene:
strbp
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX974297 [+]
aataaagtttgtatccagaatgagctccttgcccttccgtataattgtgctcacagacgctgcttgcctgttccaaaatgagtgctgctgggccaaatga
cttggggaagccctggagccacggctacctccagaccaggctgaaactccagggttcccgagcgccctgtgtcaataggcacggcatacttgctgcttgc
atctaaagaatcaggtgcacatgtcacctctcatgctgaacactgtatatatatatatatatatatatatatatatatatatatatatatacacacacac
acacacacacacactcatgatcatgaacattttacattttctttttttttttattgtgcatttttatttatttttttaaacacacagcacaaaacaacat
attaaaatacaccaacagaatagtagaattctgggtcaagtttggacattttctgtttcctgctacaataataatctataaatctgtgcttttgttgtgt
gattaataccttaccactgagctttttttatgtgcccttcattccagcatgctctccgattactggccttcggacagatatacaaagtcttacagatgga
ccctctactttctaataaacctttccctaaatattcctggtcaaattcagacagagaaggttctagtttaaaaaggccgtttgatgacggatcatgtgac
gaaaaagatccaaataagaaaatgaagagaaatttgcgcaaagtcttagacagcaaagccatagactccaaccagccaatgaatgcactaatgcgac
3' EST: CX974296 [+]cgagagcagagagtgcagcactggcctttgctaccttcttgtttggccctgctcccctaaatttctgagggtctacctcaacctccataacaaatctctt
gtcatgactccctccactctctgatatcaattcatatttcaggcctcggcgcttctcattgagctccatcactggatttttgccactggctgtaagaatg
ggaccctgagtccgtacctccaaagtgttgttgtctgcgttggagcttcctgtaatattgctggagctggaaaagaccgtatcgttcttgccttcactgt
ctgacttctcatctgaacttaagcactccatatctgtatcaaaaccagtagggtaacccatggcttgcaaaaccttcactgctacatgaagtttggctgt
cttcttagatggcccagaagcttcatgtgttgtgccatctacatccacagacatggtgaagactggcgcatgaactgggccggactgtgacaagagtttg
tactgcagaccaggtcgaatttggttaagtcgcattagtgcattcattggctggttggagtctatggctttgctgtctaagactttgcgcaaatttctct
tcattttcttatttggatctttttcgtcacatgatccgtcatcaaacggcctttttaaactagaaccttctctgtctgaatttgaccaggaatatttagg
gaaaggtttattagaaagtagagggtccatctgtaagactttgtatatctgtccgaaggccagtaatcggagagcatgctggaatgaagggcacataana
aaagctcagtggtaaggtattaat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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