XB-CLONE-3551785
Clone Name: IMAGE:7714937
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Gene:
XB5758585
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX974327 [+]
ggagttgtagctgcctctcctttggttgatgataaaattatcggggaatatgaatgtgcccctcactcccaaaaatggcaggtttatttcacctataaag
gatatccctggtgtggagggtccttaatctcatcgaggtggatcatttctgccgccagttgcaaccagtcacccaagtacttgatcgctcaccttggaaa
gcatgatattaccagggaagaaggaacagaacaacatatccaggttgagaaaacctttccacataacagatacctgggcctttctgatagcaataatatc
atgttggttaaacttgctgagccagctcaattcaatcagtttgttcagcccatcaaagttgcaagcagctgcccaagagaggggaaagtttgtcaggtgt
ctggcttcggaaatctgaattcatatgctgagaagtaccctgatagactgcagtgtctggatttacctattctgcctgaatccagctgtgacgcttactt
cagtcccaaaaaaatgcatacaaatttaatgtgtgctggctttgcacaagacgacaaggactcctgccagggtgatgccggagggcctctcatttgtaaa
ggggagctctacggaataatattgtggggaaatgaatgctcgggaagaggtattccaggagtgtaccttanagtctgtaactttaccaactggatgcaga
atattattaacaacaactganacagctcagggcctgaaaggcacttactatacatcaccatttattgtagcatgaaataaaataccacatattgtatgca
atacacaaacatagataccagtgctattctgtgtgtaaaatgtatccaataaaacaactgaagcatcaaaaaaaag
3' EST: CX974326 [+]gaggtttttttgatgcttcagttgttttattggatacattttacacacagaatagcactggtatctattgttaggtattgcatacaatatgtggtatttt
atttcatgctacaataaatggtgatgtatagtaagtgcctttcaggccctgagctgtttcagttgttgttaataatattctgcatccagttggtaaagtt
acagactttaaggtacactcctggaatacctcttcccgagcattcatttccccacaatattattccgtagagctcccctttacaaatgagaggccctccg
gcatcaccctggcaggagtccttgtcgtcttgtgcaaagccagcacacattaaatttgtatgcatttttttgggactgaagtaagcgtcacagctggatt
caggcagaataggtaaatccagacactgcagtctatcagggtacttctcagcatatgaattcagatttccgaagccagacacctgacaaactttcccctc
tcttgggcagctgcttgcaactttgatgggctgaacaaactgattgaattgagctggctcagcaagtttaaccaacatgatattattgctatcagaaagg
cccaggtatctgttatgtggaaaggttttctcaacctggatatgttgttctgttccttcttccctggtaatatcatgctttccaaggtgagcgatcaagt
acttgggtgactggttgcaactggcggcagaaatgatccacctcgatgagattaaggaccctccacaccagggatatcctttataggtgaaataaacctg
ccat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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