XB-CLONE-3551767
Clone Name: IMAGE:7714843
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Gene:
XB5961956
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX974339 [+]
ccgaggggcccttctgcattaaccatagcatgtggtacagtgagtgtaagttcctttggcagtgccgcacagttctactctttctgctgtgggagggaat
gaactgtcaggaacagacagaggctctgccagggtttagcatagaggccccagcaggggtgagagtgcagaggggactctgtgtgcgcatcccctgtagc
tttactgttagtcctggttataccttaaccagagatgccattggaatctggtataaaggaacagaaagtccgacagttgcagcttctactgactcctctc
agttccctgatacaacaaacgggcgctttatattcactgggaaagtgagtgccggagactgttccttctctatcagtgatgcacaaccaggagatactga
ccggtaccagttccgacttgaggatagagatggattaaaatttagctaccttggtacccaaccatctgtatctgtgacagatcccaaagaaacgtgtttg
gatccagtagtgatttctgcaatggtggttggaaatattgtcattttagctctgatgggcctgggaatcttctggtacctcaggaaaactaggaatgtgc
aagagacaggtgaaataaaagaaggtcctaagccaaaagctgaagagcctacatatgagacaatgaataatcaaacccagaatacgtacagtgaattgaa
tgtatcgaccatcagcaggattcactgagtcatttctgcctccccccnacaaaaacactttacatttaaaatcatttaaaaatggcattgattattaact
ataaagcaatattgattatggtttgtatggc
3' EST: CX974338 [+]gaggttactggtgcaggtactgggaaacaattaatcagtagttcgtttaatcagtattctataagttataaatagaaaaaaattgttgccacagatgcaa
tttagacctgtgttactagatcagccccagtgttaatattctataatgataaagattagacgatacagtagagtgcataaaatgatataaagatacaagg
atgttatactcggccatacaaaccataatcaatattgctttatagttaataatcaatgccatttttaaatgattttaaatgtaaagtgtttttgttgggg
ggaggcagaaatgactcagtgaatcctgctgatggtcgatacattcaattcactgtacgtattctgggtttgattattcattgtctcatatgtaggctct
tcagcttttggcttaggaccttcttttatttcacctgtctcttgcacattcctagttttcctgaggtaccagaagattcccaggcccatcagagctaaaa
tgacaatatttccaaccaccattgcagaaatcactactggatccaaacacgtttctttgggatctgtcacagatacagatggttgggtaccaaggtagct
aaattttaatccatctctatcctcaagtcggaactggtaccggtcagtatctcctggttgtgcatcactgatagagaaggaacagtctccggcactcact
ttcccagtgaatataaagcgcccgtttgttgtatcagggaactgagaggagtcagtagaagctgcaactgtcggactttctgttcctttataccagattc
caatggcatctctggttaaggtataaccaggactaaccagtaagctacaggggatgcgcacac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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