XB-CLONE-3551525
Clone Name: IMAGE:7715057
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Gene:
slc25a3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX974504 [+]
ccgaaatgtattcctccgttgtgcagttagcgcggaccaaccctttcaatgctccccactttcagttggggcaagattctgtaatatcaaggaaaggcaa
tatcccccaaacaaagccgacccgtcagcttgctgctgctgctgttcaagaagcaggtgaattcagttgtgaatatggctcaggaacgttttatgcatat
tgtggctttggtggcatcctgagttgtggtctaacccacactgctgtcgtaccactggatttggtcaaatgtcgtcttcaggttgaccctcaaaaataca
agagcatcttcaatggcttttccattacccttaaagaagatggtgttcgtggccttgcaaagggatgggctccaacgttcattggatattcaatgcaggg
cctgtgcaagtttggtttctatgaagtgttcaagatactgtatgctaatatgctgggcgaggaaaattcctacatgtggcgtacttctctttacctggct
gcgtctgccagtgctgagttctttgctgatattgctctggctccaatggaagctgcaaaagtacgcattcagacacaaccaggctatgctaatactttaa
gacaagctgctcccaagatgtatgctgaggaaggcatctgggcgttttacaaaggtgttgctcctctttggatgaggcagattccatacacaatgatgaa
atttgcttgctttgaacgaactgttgaagctctctataagtatgttgtgcccaaacccagaagcgaatgttcanaatctgagcagttggttgtcactttc
gtggctggatatattgctggtgtcttctgtgccattgtctcacatcctgc
3' EST: CX974503 [+]gtgtttgggatggatatttagttctgtacattaaatctttaaccaagagtttgttaagtttaaattacataaatgcaataactctcatctgtttatttca
acagataaatacagcggtaaaaccaagttacaatgtgtaggctatgcggcaaggtgagagcacaaccaataattacatctgaaacaaccataggtcctat
tttttagttgaatttataaaaaacgccttttgaaaatgttggagtttgcatgatttgagtccatgtttactgagtgagaccaagcttcttcttcaaggat
tctggcatctcagggggaggaggacgaggaagtctgaagtaaaccttcacagagtcatagataaaccactgcaaagcagtcaaggtaccaatcatgatga
tacgggcagtcaaacccttccagacacctttaggcccaagtctcttcagaacttccaaagcagtgcttcccttttccttgttcagtacagagaccacaga
atccgcaggatgtgagacaatggcacagaagacaccagcaatatatccagccacgaaagtgacaaccaactgctcagattttgaacattcgcttctgggt
ttgggcacaacatacttatagagagcttcaacagttcgttcaaagcaagcaaatttcatcattgtgtatggaatctgcctcatccaaagaggagcaacac
ctttgtaaaacgcccagatgccttcctcagcatacatcttgggagcagcttgtcttaaagtattagcatagcctggttgtgtctgaatgcgtacttttgc
agcttccattg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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