XB-CLONE-3551456
Clone Name: IMAGE:7715063
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Gene:
fth1.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX974551 [+]
ccatcgattgaattcggccgaggctttttttagacaaaatctctctctctctcttgcattactcttttttttgtgacttttttgcgccaccagcaaacac
cgaaacgccgccatgcaatcccaggtgcgtcagaacttcaacagcgactgcgaagccgccatcaaccgtatggtgaacatggagttgtatgcctcctatg
tctatctgtccatgtcttactatttcgatcgtgatgacgtggcactccaccatgtggccaagttcttcaaggagcagagtcatgaggaaagggagcatgc
cgaaaagttcctcaaataccaaaacaaacgtgggggccgcgccgtccttcaggatatcaagaaaccagagcgtgacgaatggggtaacaccctggaagcc
atgcaggccgctctgcagctggagaagactgtgaaccaggctttactggatctgcacaagctggcatctgacaagactgatcctcatctctgcgacttcc
tggaatctgagtacttggaggaacaggtgaaggccatgaaggagcttggagactacatcacaaacctgaagcgccttggggtgccgcagaatggcatggg
cgagtacctgttcgacaagcacaccctgggggagagtagctaagcgcactctcagggtggaaacaaccagtcagctctctgtttcaaaatactgtctctt
tactatctgtgtggattacaccatagctcccatgcccctgttgacacctttttatcttgaggatgatgggaataaatctgtgctattactgttctgaatc
atgcagaagctgctgtgcttgtcaacatgtttttcataaagtttt
3' EST: CX974550 [+]ttgacaagcacagcagcttctgcatgattcagaacagtaatagcacagatgtattcccatcatcctcaagataaaaaggtgtcaacaggggcatgggagc
tatggtgtaatccacacagatagtaaagagacagtattttgaaacagagagctgactggttgtttccaccctgagagtgcgcttagctactctcccccag
ggtgtgcttgtcgaacaggtactcgcccatgccattctgcggcaccccaaggcgcttcaggtttgtgatgtagtctccaagctccttcatggccttcacc
tgttcctccaagtactcagattccaggaagtcgcagagatgaggatcagtcttgtcagatgccagcttgtgcagatccagtaaagcctggttcacagtct
tctccagctgcagagcggcctgcatggcttccagggtgttaccccattcgtcacgctctggtttcttgatatcctgaaggacggcgcggcccccacgttt
gttttggtatttgaggaacttttcggcatgctccctttcctcatgactctgctccttgaagaacttggccacatggtggagtgccacgtcatcacgatcg
aaatagtaagacatggacagatagacataggaggcatacaactccatgttcaccatacggttgatggcggcttcgcagtcgctgttgaagttctgacgca
cctgggattgcatggcggcgtttcggtgtttgctggtggcgcanaaaagtcacaaaaaaaagagtaatgtcagagagagagagagattttggtctaaaaa
aag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: