XB-CLONE-3551390
Clone Name: IMAGE:7714974
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Gene:
apoa1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX974594 [+]
ctctcaggaacacaggcaaagtatttcccttggcagcatgaagagccccacactccagcccagcaggtgcgggaagtcttagagagctacgtacacaaag
ttcgggacttgggcagagaagctgtgagccaactggagacctctgatctcggaaaacaacttgacctgaagatcactgagaagcttgacacctttggtac
caatgctatgaacctgagaaagcaaataaatccctatgtagaaaatatcagggaacaggtgtccaaagagctagaaaaggaactcccagtaatcaaggag
aaaatccgccccatcctggaagctttccagaaacgttgggctgatgatgcaaaggcttaccaggagaaaatctctcccctggtagctgagcttcaaaagc
aaaccaagaacaacctggattccttgtataaaaagatcactcctattgctgaagagataagggacaaagtgagagtggatgttgactcccttcgctcaag
gctcacaccctacagtgaagaggtcagggtaaagctggtcgagaaactagaagagcttaaggccaatgcaggtcccagagctgaggagtacagagcccaa
ctgtcacagcatattgagaatttgaaggagaagtatggacccctggcccagagcatcagagaacgcctggtaccccatgcagaggaggccagaaacaacc
tggcaaaaatttgggaaaatctgagagccaagctccaaagacagggtgcctaanaccttcaccttctatctgtgaagatgtatgttggtgcttcaaacct
catccaaacttctcgctcacacaaccacctattatctcaacttatactattaccatgatgctggcctg
3' EST: CX974593 [+]aactggagagaggacatgtgccctcagggcaatttagatgaaatatgtaaacaataaaagatatgcagcaattcatttcatatcaatattggttgccctc
ttggagataatgtaagggctagcagcatgtaaacatgtcctctctcaggccagcatcatggtaatagtataagttgagataataggtggttgtgtgagcg
agaagtttggatgaggtttgaagcaccaacatacatcttcacagatagaaggtgaaggttttaggcaccctgtctttggagcttggctctcagattttcc
caaatttttgccaggttgtttctggcctcctctgcatggggtaccaggcgttctctgatgctctgggccaggggtccatacttctccttcaaattctcaa
tatgctgtgacagttgggctctgtactcctcagctctgggacctgcattggccttaagctcttctagtttctcgaccagctttaccctgacctcttcact
gtagggtgtgagccttgagcgaagggagtcaacatccactctcactttgtcccttatctcttcagcaataggagtgatctttttatacaaggaatccagg
ttgttcttggtttgcttttgaagctcagctaccaggggagagattttctcctggtaagcctttgcatcatcagcccaacgtttctggaaagcttccagga
tggggcggattttctccttgattactgggagttccttttctagctctttggacacctgttccctgatattttctacatagggatttatttgctttctcag
gttcatagcattggtacaaaggtgtcaagcttctcagtgatcttcaggtcaagttgttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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