XB-CLONE-3551368
Clone Name: IMAGE:7714976
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Gene:
acox2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX974610 [+]
tgaatccggacctggccagtgagagggccgccccgtccttctgtgtggagtccctcactaacctgctggatggctctgtggagaaaacacgcgtgaggag
atatgtggttagcaccatcctcaaagatccagttttcagcaaagaaaatcactattttaaaacgcgccaggagcgatacgaaggggccatacgcaatagt
tttcatctgaagcagaaaattaaggaactgggttggagggaagatggaccagaaggtgaagttatttacagggccatgggaggagaacttgccctgaaca
tccacggcgtcttcataaaatctattttggcactggggacagatgaacaggttgccaaatggattcctctagcaaataattgcaatatattgggaacata
tgcgcaaaccgaacttggacatggaacatatctacggggactggaaactacagcaacttttgacatttctacccaggaattcatcatcaacacaccccaa
atatcagccacaaaatggtggcctggagacttgggcaagacttgcacacatgccgtggttctggcccatttgatcatcaaagagaagaattatgggatgc
acccctttatagtacaggtccgaagtctgaaggatcattcaccgatgccaggaataacagttggggacattgggccaaaaatgtgttttgaacaaataga
taatggctttctcatgatgaggaatatctgtgttccaaaggagaacatgctgagcagatactctgaggttctgcctgatggaacgtatgtgaaacgaggc
tctgataaagataatta
3' EST: CX974609 [+]caaaggctgaagtgggatggatgctgagggctgtatttccttgccagagcccattgggatctaccacctccaatgttttctatgcattttgtggccaggc
atctaacccagctaggctattatacctgagcagtgtaagtgcaaataaaagatgcaggataaggcttattattattattactattagctgtttgacatca
taattatggcaacactcccggggcccagtgctttaaatgttaggcaataagaacagcagggcgaggttctctttatagttttgatatattactttgtaaa
tgtggtttcaggtaattctcaaagacagcattcaccttcttattatctgggtttttctgagcgcactcaaggagattgtaatacacgttcccatcataac
tgccaagagctgacagaagctgctgatccgcataatcgaaagcatcaaccagcaaaacagcatctctccgtataagtgacaggaggcccaggtatgcctc
agtgagcatctccagctgcttgccagacagatgcccatcctggaggaaatcaccagagttggtgaatattccatgtagtgcatgaaggtcacagaggctc
tttagtactttctggatttcaggaaaacttgataaggagtccaatacatcagcaaacagcttcacaataatgtaatgcgtatgagctattgaggccttta
caagctccacggatgtgctgttccaggcagcgtattgttccattccagactgaaccaggttgcgcatcttaaaggcagca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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