XB-CLONE-3551309
Clone Name: IMAGE:7714886
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Gene:
ilf3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX974650 [+]
tttcggttcactccagatactgcctgaatgatacgagacatacaaaaaaagaacgcatcccactgtattcttaaaataaattcttcacttataaaacggg
gaaagaacaaaaatgagggaaaaaaacaaaaaacatttcaaaacactaacgcgttaaaatacataaagcttgttaagtcacttcacagaattattttaac
gatagggaaatggaggtgaaaatggatgaatgttctgctcaggattaaaacttcccttacctagagaacaggctaaataccctctctcccagcctctgcg
cgcagcagtgaaagtgccaaatgcaagcgcgggacagtgaaaatggcgccacagagccgttaaaccagtcggggggttagtgcaaaaccttatccgcttg
tgtttggggttaggatttggcttagagtgtacaaatcctaagagcagaaaggttctggcagccatgtttaaaccttccttgacttccacacgtcactatg
gttccaagttgaacaagtacaggacaagggcggaaggaaaccaaacttacacactacagacaaggctgcttccgggcgcggtggagagttccgacaatat
ggcggagggacagatttgaggagaaagtggaagagaccgtttagaagggagcagagtgaggccgttacataaaagcactgcaacaaggaggagggggaag
agcagaacatccccttaacgatgtcttaaaacgagaagtggtaagtaacaaaagcttggggctcccctcgcagggagactgggggggctagagagtttat
ctgtactggtagctca
3' EST: CX974649 [+]cagctaatgtatagtgttgcaggagcagttaatatcaggtatagttgcatagcttgtctgttgtagagctgtggaacactccattaatagggggctgtgc
tatcttgtgtaggggatagtactatatatgctgtaggctttcttttggcctctggttatttgcctgttctgctcatatctgttatcttcacgtgatacta
ttcaaattaactgcttttgaaaggctgcagtttttcacgagttaatatttttgagatattaagtttgaactgtttttgtggtaaaagcatgcagttttgc
ttaagcgttcatgacttgctgactgacgacacctttttcatatcatgtactgtatagtggtggtgtgtattgatatatcctgacagggccttggtgtgcc
tggaaggctttggtacagtgaccgcttgataccgcctaatttaccttacagcttcagcagagcttctcttaaatacttctggtcagttctatagcaacgg
aggagcttcaggcaacgcaggaggaggtggtgcaggaactggtggctacagctcttactatcagggagatggttataatgcgccaactccaccaaaacct
tttgtaaacaagaagccaccccctcctcaacagcagcaacaacagcaacaacaaccaccttcacagcatgccagcaaccctcccaaccagcctataacca
aggctaccagggccatcaagggcagcaacaacaacagcagcaacaacagcagcagcagacttacaatcagaaccagtacagcaactatgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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