XB-CLONE-3551262
Clone Name: IMAGE:7714938
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Gene:
hsd17b10
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX974684 [+]
ccatcgattcggcatcgggccttggccgggctactgtagaacgtttggtgcgccaaggagccagcgctgtcatcttggatttgcccaaatcagaagggaa
gactgttgctgagtctttaggcgagaagtgtgctttctctcccactgatgtgacttctgaggcggatgtgaaaaatgctttggaactggcacgtacaaag
ttcggtcgggtggacgttgtcgtgaactgtgctggaatagctgttgctgcaaaaacatacaacctaaacaaacagctgccccatagtttagaggacttcc
agagagtaatcaatgtgaatatcgctggcacttttaacgttatccgcctggcagctggcgagatggggaagaatgaccctgatgaggatggacacagagg
ggtgattgttaatacagccagtgttgctgcttttgatggacaggtggggcaagcggcctactctgcctccaaaggaggaattgttggtatgactctgccc
attgctcgagacctggcacccatggggattagagtcgtaactattgctccaggattatttgcaactcctctgcttgttggacttcctgaaaaggtgagaa
atttcctggccaagcaagtgcccttcccaagcaggctgggtgaccctggggagtatgcgcaccttgtgcagtccattgtggagaatccaatgttaaatgg
agaggttattcgacttgatggggccctccgcatgcagccttaatggaaaggactgtatgattcagtgggaccagtcaggctactggttcatgccagcagc
aaaacccaggatgtatatccctgtaattaatagcagtgattaagtgctttattccctggagtgcaggtaaagcgaatggagttattaatta
3' EST: CX974683 [+]aagagacagcgacagaaaggccaattttcttctcacagtttaatcactgttacagttttcattgttagaaacttttgtagaacatggttttggctgatct
tttagagattccaggtttggtaaactttcttggtcagatcacaggttaattaataactccattcgctttacctgcactccagggaataaagcacttaatc
actgctattaattacagggatattacatcctggttttgctgctggcatgaaccagtagcctgactggtcccactgaatcatacagtcctttccattaagg
ctgcatgcggagggccccatcaagtcgaataacctctccatttaacattggattctccacaatggactgcacaaggtgcgcatactccccagggtcaccc
agcctgcttgggaagggcacttgcttggccaggaaatttctcaccttttcaggaagtccaacaagcagaggagttgcaaataatcctggagcaatagtta
cgactctaatccccatgggtgccaggtctcgagcaatgggcagagtcataccaacaattcctcctttggaggcagagtaggccgcttgccccacctgtcc
atcaaaagcagcaacactggctgtattaacaatcacccctctgtgtccatcctcatcagggtcattcttccccatctcgccagctgccaggcggataacg
ttaaaagtgccagcgatattcacattgattactctctggaagtcctctaaactatggggcagctgtttgtttaggttgtatgtttttgcagcaacagcta
ttccagcacagttcacgacnacg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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