XB-CLONE-3551090
Clone Name: IMAGE:7714908
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Gene:
cd74
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX974804 [+]
ccatcgattcaattcggccgagggtcggaaacagacagccacggatgtcctgtaacaaagggagcctggtgacagctctaacggtgcttgtagccgtgct
tgttgccggacaagccgttatggcctatttcatcacccagcagaattccaagattcagaaacttgaccaaaccaccaaacacttgcagctgaaagatatg
atgaagaatcttccaggatcgcctcctgcccaaattaaatcaaagatgagaaccttcaatatccccatggcgttaaaactatatgatggctcagaaacaa
atatgaatgaactggagcatctggctcagatcgataacaaagtggaagatgctgcaaaatacatgttgctgagaggaaaccctctaaggaagtacacttt
accaaatggcactatcctggaaaacctgagagagctgaagaagtccttgactgaccaggaatggatgaactttgatgcatggatgcatcagtggtatctc
ttcttcttggtgcaaaacactggaaaggcagctgaacccctcccaccgcaaaagaatattgcagtcacaggtgcccccctgatgactgagtgtcagacat
tatcaaggatccccaccctgactggcgcttataagcctcagtgtgagcaaaatggtgactttaagccgaggcaatgctggccaagcactggctactgctg
gtgtgtttaccgtaatggaactgaggtccctgactccagaacccggtggtcaagacctgcctgcagtgatgtcatggagccagaagaaccaatttttctg
ggatcaacacccagcgatgactttaat
3' EST: CX974803 [+]gatgtaactgctcattttattaggcatggaataaggtatgtgcacagtatacagaacctgtaagtctgacagaagccgcaattaacagcagagaaaaagt
taaaaaaaaaataaaaatggcagccatatttctatcaaaacacaattagcgataaatgaatgaacaacgattttatcagtgcccatttgaattgtcaaat
catgttaatgttatcacattccccagaccgtaccttactgagacctccttaaccatacagaaactactagatttgttagtagtggcaagacgtggaggat
ttgtccatggtatagagacatagacaagccacttttcagaagtactagttagtcagcctcctggtagatacttcctttcaatcatgtgcagctacaatca
cagtgggactgatctgtgtaaccataccacttattattcctttaacatgctgctgtaacacacactgtaattcataaaaacaaacaaagtactaaacact
accttaagcctattgagaaacctgaggccagaatgccatagcaattagtaacaacataaagtcagattaataggaatcctcttgtgctgcttgtatgaat
cagggtagatactgcagaggaggttacgtgctgatccgatgctttaatcaatgggattaaagtcatcgctgggtgttgatcccagaaaaattggttcttc
tggctccatgacatcactgcaggcaggtcttgaccaccgggttctggagtcagggacctcagttccattacggtaaacacaccagcagtagccagtgctt
ggccagcattgcctcggctaaag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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