XB-CLONE-3550946
Clone Name: IMAGE:7714970
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Gene:
flvcr2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX974899 [+]
tttaaactgcaatactcaagtcagattccatggcagtctgtgcatcctggtttgcaaaagatattgatagaattcttctttggtataaaaacttaaagtc
caaaggtaaattgttcacttaggcataaaagaaccctgatggaaacatcaccagcctcaaaaatgccattaccaagcagacattttgtttaaagctgtgt
tctgtggcaagatggaatctgttatcactcttgacaggacaagagagaggaaggggtctccccttcttccacttgtggctcagcattctgattattggct
gcctcctgattggctctttgtctgcgaaggtcaggcttaatacatcctgttatgattgtccccaccaacaggaagctgcagaggaaaatatttccagcct
caaccccaaatttatccagaatttgtccctgtgatgtggtgaagatcagaccaaatacctgtgctgatacattcagtaatcctgatgatgttccctcaga
ctctggatgtgtcaattctgctgcaaactcaaatccaagtggaagatatcctgtcataaagaaccccagacatccagcagtgacaaagacgacccagaga
tagcgaagcttcaggatgaaagtgaatgtcaccattcctgccagggaaaaggcatacacaaatagcgtagtctgtttgtatgtctttgtcctgtccagcc
agagacctgtgagcaatgctccacacatgccagacactgtgatagtcaagccaatcttcccagcatttacttcttcacctggatggtaa
3' EST: CX974898 [+]gaggttttgaggtggagagcaatagaagcccttgaaattcctgtctggcaagggtgcactgtgcagtaatggtgagagaggcacctccgaatggtgtgtt
actggattctacacagtcaaacagtatggcagacaagatcattcgtccaggcacagctactttgccccagtctccagaaagtgaactgggtcagaactca
ggagatgctacaaagctgtacacaaggcgctggttcattgtgctgctctttagttcatattctttgtgcaatgcattccagtggcttcaatatggtatta
ttagtaatatcatcatgcactattatggtataagcagtctagccatagactggctctcaatgtcatatatgttggcctatatcccactaattttcccagt
ggcctggctcctggacactcgtggccttcgtgtcattgcattaattggctctgggctgaactgtttgggtgcctggattaagacaggtagtgctcggcca
gacctgtttggagtgacactttttgggcagctggtgtgtgcagtggcgcaagttttcatcctgggaatgccgtctcacatagcatctgtgtggttcagtt
cttctgaggtctccactgcatgctccattggagtatttggcaatcagctaggggtagcgattggatttcttctgccccctgtgctgataccgaacattca
agacttggatttgctttcatatcatctcaaacttatgtttattggcactgcagctgtagcgtctgtactgtttatcctcgtcatcttcgttttccaggat
gcccccaaagaagcct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: