XB-CLONE-3550530
Clone Name: IMAGE:7715343
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Gene:
srsf7
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975180 [+]
agccttaagatttcatgctgaggcgccttgcaaatccgacattatgattctgctagaccttgaggtgatcagcataagaggccagatcccctcaagcctt
ctacatctagatctaccttaagattctttagaggccagaaaatctatgatcaactgtgatcgccgtatcagtaaaattggcttacagttggggctcccct
ccgctttagaaagaactgcagattgaccacattcccacctagaaaaaccttcatgcgtcaatcaagcctcacctggctcacatggctgtcagtttgatcg
tcgttagattgaagaacagctacagtagatgcagcgatcggctggtagatccttctagaccgtctagagcttctagaccttgggccaaagagggtcgacc
tgcagacttgcaaggtttattttaactacaaattatagtgatatttttactttggttaaatattaatgtaaaatggaactcctttaaccagtttctccta
ggtagatctctccaaggcaaataggtttcctgattattcagacactgcttaaaggtctggttaaagcaggctacactacaaaatctgcacttcttagatt
ctaagtaattccatgttttcattctccaggacaaggtctcggtctcactcaagatctaggggaagaagatatacccgttcacgtagcagaagccgtggaa
gaaggtctaggtctgcttctcctcgccgttcaagatctgcatcaccacgcagatccaggaatgccaccccacgtagttctcggtctggctctgttaaaag
atccaggtctctatccaggtctcgctcaagatccagatcggtgtcacgtcccagaagcagatcnaagtccagatctgcatccccaaaaagagtcgctcac
catc
3' EST: CX975179 [+]ctgaatgccaaagtgctataaacatttgcaatgaatggagagctcttaacacaggatgctaattcaagctgcaaatgccacactgagcagtaatttcccc
ccactttatacccacagtccataaaaacataaataaaccctccaatgtgaccagtaagcctttatcaggtaaccaaattaaaaacaaaaaaaaaaaaaca
aactccaacagttaaatggttgtcaaagctgcatagagtttcctatatacaaggggtaccccatcatgtacccatcacctcacaatttcaaacagcaagg
tctgctttaagatagtgaagactcactagtcaagacaagctatttaatgttcgacaattctgaaatacaaaaatttgttttatagacaagatacaacata
cattgtatgctagcctagaagttcaaaccaaaaaattttgaaggtgaaaaacttggctgcagcatattgctgtcaattcttgtgaagtattctagccatt
tctttctgggcttatgcttcttcgcggacttctggatggtgagcgacttctttttggggatgcagatctggactttgatctgcttcttggacgtgacacc
gatctggatcttgagcgagacctggatagagacctggatcttttaacagagccagaccgagaactacgtggggtggcattcctggatctgcgtggtgatg
cagatcttgaacggcgaggagaagcagacctagaccttcttccacggcttctgctacgtgaacgggtatatcttcttcccctagatcttgagtgagaccg
agaccttgtc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: