XB-CLONE-3550495
Clone Name: IMAGE:7715585
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Gene:
cpa1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975206 [+]
ccgaggatttcagtggacaacaagtcctccgaattttccctagtgatgaggctcaggtgaacctggtgagggcgctggaagatgaggagtatttacagct
cgatttttggacaagcccctcaagagttcttttacccattgatatcagagttccactccaccatctgcaatcagtgaaagtctacctggagtccaacagt
atccagtacagcatcatgattcacgatgtacagaacttgctggatgaagagcaacgcgatatgagacgtaatcgtgcctctgaggatggaaggtctacta
acagcttcaattatgctgcctatcatacatttgatgagatcaacgcctttatcgacaacttggttagtgaaaatccaaatttggttagcaaaatacagat
cggttcaagttatgaaggccgtcctcttaatgttctgaagttcagcacaggtcccaaccgcccagctttctggattgacactggtatccattcccgtgaa
tgggtcactcaggccagtgctgtctggtttgccaaaaagattgtcacagattttggtaaagatccatccttgacaaatacactgaaccaaatggatatcc
tcatcgaaattgtgaccaaccctgatggctttgtttacactcataaatcggatcgtatgtggcgtanaaccagatctccaaattctggctcactctgtgt
tggaactgaccccaacagaaactgggatgcaggttttggaggtcctggctctagctcanatccctgcactgaaacctacagaggacgtgctgctcattct
gaacctgaagtgaaagccattgtggactttgtgaaaagccatggcaacatcaaaggctttgt
3' EST: CX975205 [+]aatgtgaacaaaaacacctttatttactccaaaaggtgtaaatgttgtttaataaaggttatcacgggtatgctcaataagtatagtgagtgcaagccat
gtctcctctgcagttggaatgatttggttagctggtagtgcaaatccatagcgcccagtatcacgcagctcaaagctgtaggaatgcttaattccttgat
tgtaggtccagtcaatggttcccccgcttgcttggtagatggtactgatgatgctgccataggtgtaagctgtaccgtacagtgaagccaaggcagtaat
agctttctttgacacagcattcagctcatcgtggtcgggtactcgggtattagtgtatccgtatgggaagagaagcatctgggagtagctgtgaatggag
acaaagcctttgatgttgccatggcttttcacaaagtccacaatggctttcacttcaggttcagaatgagcagcacgtcctctgtaggtttcagtgcagg
gatttgagctagagccaggacctccaaaacctgcatcccagtttctgttggggtcagttccaacacagagtgagccagaatttggagatctggttttacg
ccacatacgatccgatttatgagtgtaaacaaagccatcagggttggtcacaatttcgatgaggatatccatttggttcagtgtatttgtcaaggatgga
tctttaccaaaatctgtgacaatctttttggcaaaccagacagcactggcctgagtgacccatt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: