XB-CLONE-3550492
Clone Name: IMAGE:7715251
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Gene:
fth1.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975208 [+]
ccgaggttttagaccaaaatctctctctctctcttgcattactcttttttttgtgacttttttgcgccaccagcaaacaccgaaacgccgccatgcaatc
ccaggtgcgtcagaacttcaacagcgactgcgaagccgccatcaaccgtatggtgaacatggagttgtatgcctcctatgtctatctgtccatgtcttac
tatttcgatcgtgatgacgtggcactccaccatgtggccaagttcttcaaggagcagagtcatgaggaaagggagcatgccgaaaagttcctcaaatacc
aaaacaaacgtgggggccgcgccgtccttcaggatatcaagaaaccagagcgtgacgaatggggtaacaccctggaagccatgcaggccgctctgcagct
ggagaagactgtgaaccaggctttactggatctgcacaagctggcatctgacaagactgatcctcatctctgcgacttcctggaatctgagtacttggag
gaacaggtgaaggccatgaaggagcttggagactacatcacaaacctgaagcgccttggggtgccgcagaatggcatgggcgagtacctgttcgacaagc
acaccctgggggagagtagctaagcgcactctcagggtggaaacaaccagtcagctctctgtttcaaaatactgtctctttactatctgtgtggattaca
ccatagctcccatgcccctgttgacacctttttatcttgaggatgatgggaataaatctgtgctattactgttctgaatcatgcagaagctgctgtgctt
gtcaacatgttttca
3' EST: CX975207 [+]tgaaaacatgttgacaagcacagcagcttctgcatgattcagaacagtaatagcacagatttattcccatcatcctcaagataaaaaggtgtcaacaggg
gcatgggagctatggtgtaatccacacagatagtaaagagacagtattttgaaacagagagctgactggttgtttccaccctgagagtgcgcttagctac
tctcccccagggtgtgcttgtcgaacaggtactcgcccatgccattctgcggcaccccaaggcgcttcaggtttgtgatgtagtctccaagctccttcat
ggccttcacctgttcctccaagtactcagattccaggaagtcgcagagatgaggatcagtcttgtcagatgccagcttgtgcagatccagtaaagcctgg
ttcacagtcttctccagctgcagagcggcctgcatggcttccagggtgttaccccattcgtcacgctctggtttcttgatatcctgaaggacggcgcggc
ccccacgtttgttttggtatttgaggaacttttcggcatgctccctttcctcatgactctgctccttgaagaacttggccacatggtggagtgccacgtc
atcacgatcgaaatagtaagacatggacagatagacataggaggcatacaactccatgttcaccatacggttgatggcggcttcgcagtcgctgttgaag
ttctgacgcacctgggattgcatggcggcgtttcggtgtttgctggtggcgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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