XB-CLONE-3550361
Clone Name: IMAGE:7715360
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Gene:
cdv3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975296 [+]
tgactgattagttcattttagcagttggtaaaacaatacaatgggatgcagttagaagtcagtgctactctgtgagataatgagagattttcacctgaag
gtcttgagaaaataggcctttgccgctgtcctgctagaaacctggggagaccttaacagcaggtcactatttataaacacattctagccactgattctat
ctgtataaggagctcatttactattggcctgggtgcaagagcgctttagtgcccatatacagaacacttgcacccaggccaatattgtctgtgcagccta
ctctctgcactttgcaccaccaggtcactgttgcccacaaggcagccctatacttaagaccctcaccctgctacccctggcacttcctagattgcatgtc
taaataacacccaagctagggggtattttaggggatgcacactgcccaccaccagtctcctcaaaataattgcagatccaggcaatgctgtattcagaac
aatgcacagctcttttattctagtgcacaaaaagttgtgctaatggcagaacgggccccctccagcaaaaacaatctcacgcacaatctggttggctgac
gccccgcaactcacttacttgtggtgagcgaactttagttcacagataaggggggctaccatactgcagaccctttgtcctagcccccctgtcccccatg
ctccagccacagggcctgctgcttggtagttacaccactgagctgtgcctctgggcaaggtgctaagtactaaaaacaggggtctttgggctcctttttt
tcaccttgcccactgttttgtcaaagcttctctttctttctacaccaaagacgccatttgaac
3' EST: CX975295 [+]cccaagtgaatttttacttacttgtgattttatagcagttttgcatgatatttggtgcgtgtatggcaagtcggcgagtcgaccgatgtcgcaggagtct
gctgatatcggacgactcgccgatcgggcaggttaaaagattttgattgggcgccatagaaggcacctgagcgaaatctgccgtcagggctgaatcggca
gaaggaggtagaaatcctattgtttctgcctccttatctgccgtttcagtcctgaaggttagtggcggatttaatgatctttcgtgtgatttaatgatct
tttggttgcacgaacgatcgcaaatcgccacgtgtgtggccaccttaagtctccctaaatattcattattcattacctcatgcacagatcaaaataaagt
attgtctgctgacccctcattttgtggagatttaggttaaattaaatggagtttggtttgttatataatgctattccttttcagatgatgcagttgagtt
acagtagtaggtacacaatatgggcaaaagtatatgtacaccttctttattaaagtcacacctgtcattgccaacaccggtgccactacatcatacaatc
acattcttgggaattccatgcttcttactttgcggcaacagtttggggaatgtcctttctagggatgcactaaaccctccatttcagtattggtggaatc
cccgaatccttggatatggattgagctgaataccgaactgaatccagacctggaagaggaagccagaagtgtggtaaataaagtggaaaatgttggagga
aaaatattttttacattaca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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