XB-CLONE-3550264
Clone Name: IMAGE:7715416
|
Gene:
nipa1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX975360 [+]
ctaggactgagtattgctgttttatctagtttattgaatggatcaactttcgtactacaaaggaaaggcatcctcagagctcgcaggaaaggtggctcat
acttagctgacattatctggtgggctggaactattacaatggcagttgggcagattggcaatttcttggcatacactgctgctcctgcagtgcttgttac
acccctgggtgccctgggtattccatttgggtctattttagcttcataccttctaaaagaaaatcttaatttcctggggaagctgggatgtctgctttgc
tgtgtgggttcggttgttttgattatccattccccaaagtctgatggtgtaacatctctattggaactggaagaaaagttcacaaatccagcatttatga
cctacctgttggttgtacttcttatgctatttatgctgatattctggatagctccaagccaggggcacagaaacataatggtttatactggtatttgttc
cttattgggcaccttcactgtgccctgcaccaaaggtattggtctggtggctcaggaagcctttgcaagtaattctaccaactcaagagcgctttacatc
tttgtgactctcctagctgtactgggctgcagcatcctcattcagtttagatacatcaacaaagcactggaatcatttgactcctgcatatttagtgcaa
tctattatgtggcattcaccaccctagttttgttggcaactgccatacttttccaagagtggacaaaagtgggagcagtagactccttggctattgtatg
tggattcacgaccatgtcaacaggagttgtccttatacagatgtttagggagttcagtatcaaccta
3' EST: CX975359 [+]ggaattgtaaaggccacagcctttatttatacaaatagtatttgcataacatgtaaatctctaaccaataacagtaacaggggaatgcttgccactgcac
agaacacaccaccggagtgaacgggcccccctgccatgtcggccaccaggtgcagatgcactgtggagggcaagtggtagcaagtgggtctcactctgtg
ccaacgactcctgggcccaatacccagggaggtaatgacccatactccgctaggcagaggaacctgagccccttaccacctggtaagcattccctaccgc
tagcacccactgctgtgactgaaacaagggagggtgggcggaacgttgccctttgtgctaatacatatggcgggaaacggaactccctgcaccctttata
tcctctcctagccactcctctcacactgcccttgctactgcccagctttttagtcctccctgccttcctagcccaggcaaggctccccttccgttaaccc
tttggtgtccagggggcatcattctctgcaagaataaatcttttaggacacactaagaagaatttgctgtgaaactgcagggccattgtattggctccca
gtgcaggcacaacatccagattttttgaacatggggcggactcttggcatgcatttttcccacaggccaagaatctgtttcatgtggcattatcctaagg
gctaggtgacacgggatggattgtcagcctgcagataaacgtaggctgacaatacgctcctacacccaaaaatccttctaggtgtgcctgcatct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: