XB-CLONE-3550155
Clone Name: IMAGE:7715378
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Gene:
etnppl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975435 [+]
ttttaacatgataagtgctttatttgtattcatttgatgttatgtagtaccattaatacttgacattgatttctgttgtctaatatttaaaaactatgac
tgaaacactcccaatctgtgactttcagtttcaccaatttcctatgccaaacactcaaatatctagatttaatggtcaagaacaacaaaatccaaataac
tgcaagactggataaaaacatctaaaacacacagaaacaaatctagtttatttgactagatgtgcagaatttaatgaataagcttttatttcttattatg
ttttgaatctagtttatagagaacaaatgatatactgtagatatgcatggcatggatttagccaagcagtaggatctggtataatgtgattatggatatc
tgctcttctggcactctttcatagtcattcttgtcaagtgctgttatacatctgaaatgttctgacactttaagaaaagaagcatacgcatgtttaccta
gttttaagaaaactgctgatcccccatgtagcttctcattttaaaatataaaacattatagcaccttgtatgttgacaaaaaagaaaataccataggcag
tgagaaaatgcccccttatgctattctatggggctcatattcaagtaaaagggatataggaatcatgtacacagggatttcaggctgctacatcagatct
gtagggacccctaataaaaagtaaaataatgtagcgagtggaaaagggttacttatttgagcaagagctaaaatcttggtgtatctaaacagttggaaa
3' EST: CX975434 [+]aaaacttcccagccacagcagcagagatgtgtgaggaggagtactgcaaggaaaagatcattgagatgagaaaaaagcacattgggccttcgtgcaaagt
gttttttgcaaatgaccctatcaagattgtgcgtgcaaaaggacagtacatgtttgatgaaaaaggagagaaatacttggactgcataaataatgttgct
catgttggtcatagtcacccagaagtaaccaaggctgctttgaaacagatggaattgttaaacaccaattctaggtttctacatgacaaccttgtccggt
atgctgagtgtcttacagccacccttcctgagaagctttcagtctgctattttgtcaactcaggctcagaagccaatgaccttgcactgcgtttggcacg
acagtacagtggccatcaggatgtcataactcttgataatgcttaccatggccatgtaacctccctaattgatattagtccctataaattccaacagctg
ggaaaggatgcacagaaggagtttgtacatgtggctccttctcctgatacttacagaggaaagtacagggaagatcatacagaccctgcattagcttatg
ctaaagacgtagaggacatcattaaggaggttcataagaacaagcgtcagattgctgcttttattgctgagtccatgcagagttgtggaggtcaaataat
cccaccagcaggctacttccagaaagtgtcagagtttgtccataaagcagggggagtcttcatagcagatgaagt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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