XB-CLONE-3550059
Clone Name: IMAGE:7715530
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Gene:
ckb
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975499 [+]
ccgagggcggtttccaattaagcaagactaagtctagtgcaatcatgcctttcggtaactctcacaacaccttgaagctgaagaactctgtggatgagga
gttcccagacttgtctgcccacaacaatcatatggccaaggtgctgacaaaggatctctacgcgaaactgagggataaacagacacctagtggatttacc
gtggatgatgtcattcaaactggggttgacaacccaggccatccctttattatgacagtgggatgtgtcgctggggatgaagaatcttatgaagtcttta
aggagctctttgaccctatcattgaggacagacatggtggctacaagccaacagatcagcacaagactgacataaattcagaaaacctaaagggaggtga
tgatctggacccaaactatgtactcagctctcgggtcagaactggaaggagtattcgtggattcagcctcccacctcactgcagccgtggagaaaggcgt
gcaattgaaaagatgtcaattgaagcacttgctagcttggatggggacctaaaaggaaaatactacgctcttaataccatgactgatcaggagcagcagc
agcttatcgatgaccacttcctgtttgataagccagtttctccactcctcttggcctctggaatggcccgtgactggcctgatgccagaggaatctggca
caacgacaacaagaccttccttgtctggatcaatgaggaagaccatcttcgtgtgatctcaatgcaaaagggtggcaacatgaaggatgtgttccagagg
ttttgcactggccttacaaagattgaagct
3' EST: CX975498 [+]aagcaggttgggtccccagggtcttggagtgtagctatttcatccaataaattagaaagcatggccaataaagtgcagttatttctgtgctggaatgagg
tcatcgatggactctcctttttccaggcatttttccatttcaacaaggagttttacaccatcaaccaccatttggaccagttcaacctcagagaaaccca
gacgatcagcattggagatatcaaacaccccaccaacggctgcagtgtctacaccacctgttcctctcttctgtagtctaagtctcttcaggatttcacc
aaacttctcattcttgcttaagtttggcaacttaatgtgaacaccagccctcagaccagtaccgaggttggatgggcaggtaagaacataaccaagatgc
tggttccacatgaatgcagatcctttgttcttgaagatagcttcaatctttgtaaggccagtgcaaaacctctggaacacatccttcatgttgccaccct
tttgcattgagatcacacgaagatggtcttcctcattgatccagacaaggaaggtcttgttgtcgttgtgccagattcctctggcatcaggccagtcacg
ggccattccagaggccaagaggagtggagaaactggcttatcaaacaggaagtggtcatcgataagctgctgctgctcctgatcagtcatggtattaaga
gcgtagtattttccttttaggtccccatccaagctagcaagtgcttcaattgacatcttttcaattgcacgcctttctccacggctgcagtgaggtggga
ggctgaatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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