XB-CLONE-3549987
Clone Name: IMAGE:7715346
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Gene:
vwa1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975549 [+]
ccgaggtttttgtgtcacatcagaaaggcatcggtgccagggctgctctgcccagtgcccagaaggagtaaacacattcctgccctcacatggttaaacc
ttcaaggaagggaactttactgcagaactgggtgttgctggctcttggcagggcagctgtactggagctggtgtcaggctggaatacaagaagaattaag
gctgtctgattaatgcccccaaaggatcactgagtaggatgcaggggaagagtcccttattgctgcatatgcaccctaggagttagtatgcctgtgctgg
acaagtttgggcaccagagataaagttactctgcctgcactgtctctgctatgattgtctgtctagtactttatctgcactccctcttcctggctgctgt
aacccagaccatacctgatgcagaccttgccggatttgttcctgactccgagggcgaccttatcttcctcttagacagttcaggaagtgtctcgtattat
gagtttgccaaggtcagagagtttattggtaacctcctgcgaccatttacctttggcccacaagatgtacaagccagcattgtgcatatcagcaccaacc
ctgttctggaattcccattcaaccagtatggttctagccaggagatccaaagggccatacagaatatcaagcaacgtatgggagatacaaatacaggcaa
agctctttcttacataaaggagaacctctttgatgagaggagtgggtctagggcagaggttccaaaggtcatggtgtgggtaacagatggcttgtccact
gatgacatttctcagcctatgcagcttttgaaggacatgggcgttacggtatttattgtaag
3' EST: CX975548 [+]ccatttataaaacaattttcctcttcattttgcactgtatgctggaggggaatttttctgtaacagattttcagaattcattaccagggacactctgccc
ctgggccagtgctttacgtaaatctcaatgtacactgtgcccatgataaatgcttataaagcttatcacaatcaactgtgtcattccttccaggtatgaa
gttagagcagacagactgtagtctgtccatgggaacaatgaaatactaaaatacttctgtgcagttggtcctgtacatgggcaatgttcttttgggacag
attaacataatggatgctttacatttcaatactggtggtgccagcatggtcagggaaacacagcctgccagtttgtccacagcttgtcataaaagaaatg
actggtgtgcgttccctcatatcacttcctgcaaataaaaagttctatttccttccatgaaaataccagttgttgcaccaaagcaaaagatgaaatgtgc
ttgtttggagccttgacagttactgtgaagccaatgtgagacaaacgtgatcttcgtggttccttattaacgtattttggtgaagatgctccttagaaat
tagaagttttgcttgcagatatgacattacttgcagtgtcaagagttccaggttaattggacccaactggcatctagagacaggaaatgggcctgtgaca
cggagccatgggtttgttttctgaatgaagaataaagcagcaacttcttcacttactccttttgttagcacctgtgccttctgatctctagcttatagat
acattgatgcaatagtctcctaacatcactt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: