XB-CLONE-3549958
Clone Name: IMAGE:7715540
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Gene:
ewsr1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975568 [+]
gcatatactgcacaaccaactcaaggctacgcacagactgcacaggcaacttatgggcagcagagttatggcacctatgcacagcccactgatgccagtt
acacacaagcacagacaacagccagctatggccagtctgcctatgccacttcttatggacagccaccaacaggatacactgctccagctgctccccaagc
atatagccagcccatgcagggatacggctctacaggctatgacagtaccactgcaacaactaccacaactcaggctacatatgctgcaccctctgcctac
ggttctcaacctgcatatccttcttatggtcagcagcctgccactactgcatctgcaagacctcaagatggaagcaaaccagcagataccagtcagcccc
caccaagtacagcctcatatgcccagcccagcatgggatatagtcaaagcagctacagctaccctcaagttccagcaagctatccaatgcagcaagtgag
cgcccctccatcctaccctcctacaagctactcgagctctcaaacatcaagctacgagcagagctcctattcccagccaagttcctatacccagcaaagc
ggctatggacagcaagcctcatattctcagcagagttcttatgggcagccaagtagttacggccaacaaagtggatatggccaacagagtagctaccagg
cacccccacaacagcaacaacctcctcccaccagctacccaccaccttcgtcttcctatagcagccagccc
3' EST: CX975567 [+]cacaaattgtgaatactaaagttacagacaaagaataatcataatgtgacagtgaagggaggatcttcatgtcagcattataaatatggaattataaatt
taaaacatttctggtttaaaaaataaatctggtagtaaaggtggctccgaaactagtagggtctctctcttcgttcctggcgatgctcactcttatccat
tttgctggggccacctcgtcttccaccaccaattcttccaccaagctgttccatgagtggaccagggggacctggaggtcctcctctccttcctcctcca
aatcctctgtccataccacgccctctgaagcctcctctatctcctccacgaccacctctaaacataccaggcacaccacggtccattaggcctctgcctc
ctctcatgcctggaccacctcttccacgttctcctccttggggtgggaaaggagggaaagggccatcaggctttggagccttgcactggttgcattctgt
tctccaagcaaaattctggtttccacatccggggttggggcactgccagtcaccagccctgtgctggacattaccgctaactggactacctcttggccca
cgtggccctcttggcatgaatcccccacgttcccctcctcggccacccaagcgacccatgggacctccacggagaggtggcggctgtcctctgttgtcac
gtaaaagtgacccccctctcatgctacctagcaaagactttttgcgagccaatgacactttcaccttgttcccattaagatccttcccatcacaaagctc
tatagcagtcttagctgaaggtgggtcttcaaaggacacagtcccatctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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