XB-CLONE-3549891
Clone Name: IMAGE:7715787
|
Gene:
cd2bp2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX975615 [+]
gacgaggcgggaacgacgcggattctattttacaggttacaaatctgcgggtatttaacagtaattaaagtgcttatccggaaagaacatactccggggc
cggcgcggccctcactcccaaatatacatatgttacatggaatatatacagcatatccagtagccacgcctccttaaaggggaaacaaaggtctgttttt
tttatattttctattttttcgttactttgtagttatgttacaaattctgattgtataaatcatttactatgaggattatggcacagaacctactacagcc
ccgcgccccatccctgctctcatttacaattatttcacaataataaaaaaaaaacactttttcctttgtatccaataattataagtctcgtttacaacgg
attttgtgccgctagaatggagatttattgccattaaggatgataaaccccattgtttataaaatatacatctatacacggaagtaataatacgacataa
gtgatagaattgctgtaggtttgttgtaaatgggatagaatcggatagaatgggatagaatagcatctgtgtgcttgggcagccaccttggtcccccacc
ttggtccagaattactgctctgaagaggactgcccaatctgatttcctttactgcatgttccaaagttggccatatatgcccaaatttggccacttccta
tcatggtggcactggagtgcccacattagccccatgacgcctttagcaacaagccagttttgtgctgatacctaatacctaataaaaagaagaaacggat
cctttctggcttagc
3' EST: CX975614 [+]aggctgctcatagcctgtacagagagataccataaaactatggcacatagggattcccctgtactaagcacaattcagcaggaacagtcccctaagtttg
ctcatagcctgtacagagagataccataaaactatggcagcatagggattcccctgtactaagcacaattcagcagaaacagccccctaagtttgctcat
agcctgtacagagagataccataaaactatggcagcatagggattcccctgtactaagcacaattcagcaggagcagccccctaagtttgctcatagcct
gtacagagagataccataaaactatgtgccatagttttatcgtatctctaggcctctataatttaaatgtgtctaagcccagacctctccttgcagctgc
tcctgattctcccagcattcctcaggacttgtgggtgtagttcagcaacctcacaaccatcacctctgaacctataggctggaacagatcccagtaacgg
ctacttgcccataggaatctctggcttctattctgctcagatctaccattgtctctctctattggattctccctataggaagttaatatagtgaataatg
tgcagggggtactttatttattataatatacaagtttcagtgagtcatgtgacagaaatgacatcactaggctccgattataaccaatgacatcactatg
taccgtttataaggatataatttacagcttggtcccatagggaaattacccaactgtattatatatttattggtgccccacttcagctaattaatactct
tatcattagctctagcaattagatcttattaggccagcccctatagttgttcattaataagctaatttcatattgtc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: