XB-CLONE-3549862
Clone Name: IMAGE:7715743
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Gene:
syap1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975638 [+]
tggaatatacatttaaacttgtgcagaggaaaatcttaattgattaaaatcaggtataatgttctttatggcagggtaaaacaaaaatatgggcaaaaaa
aaaaaaccccccaagaaacaaacaaatccagacaggataccatatacctttcaaatcacatagaaccaggcctcactcattttgtacgtattggaatgga
aaacaaatgcttcgttttgaatggtgaaaaagaaatcgtgttccaataatttggcaccacacacctctgtggcaaatacttggtcttttagcttttactt
gtactagagagacatatatatgatgaaaccaccggcgcctctctggcaaagactttcttcccaggctggaaataaatacattatataacatgtcagctct
acttcagtccatatcccagataaatgaataagaccgctgtgttgttacattgtgaagtgctttttaaaatagattattacagtcacaatccagaagagac
agggcttcccagaacttcagtttaatagcacttaatcttccattgctagttctaaacagtagcgccttagcagtacatagcgaaaacagccatgatctta
ttctgtggtttgcagagcctgaaggaatgactagagtaggaagagaatagtatagacacagtccaactgatcagcattacgttagtcctcttgtaggagt
tcctcaatctctttgtcccaattttcatccttcattttgtcagagtctgtcaccacctcatattcttgaaccacatcctgtaactcctttatccagtcct
ctgtttcttcttctggtttatcagcctcatctttttt
3' EST: CX975637 [+]gagaggcttgggagctggctcgggctggacccggagcaggaaacacaaaaaacagcagagaaggaaacaggaaggaatgaagaagagactagaagcgcgg
aaccaggagacggggaaaagttgaaaagcgctggggaagatgggcagtctcagcaagaggcaaatgagtcagacgaggccctcataaaccaggccaaagg
ctttggcagttaccttttaaactttgcttctgttgctacaaaaaagatatctgaatctgtagccgaaacggctcaaaatatcaagaaatcggttgaggaa
ggaaaaatcgacggtttaattgataagacgatcattggtgattttcaaaaggagcaggaaaaatttgtacaagagatgcattttaaaaagtcagaagctg
cggttgctccttgggttggttataatgaagaggaaaccattcaacaacaaatactggccctatctgcagacagacgcaatttcttaagagatccccccgc
tggagtacattttaactttgagtttgagcaaatgtaccctgtcgcccttgtaatgttacaggaagatgagctacttaaccgaatgagatttgatcttgtc
ccaaaactcgtaaaggaagatgtgttctggaggaactacttttaccgtgtttctttgattaagcagtctgcacagttaacagccctggcagcacagcagc
aagcaacaggaaaagctgataagattaatagcatgcaagatggcggtgacttagaagaaactgtaaggccaaagactccaccagtgactatcaagtcccc
acttaaaaatccagaggaagaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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