XB-CLONE-3549839
Clone Name: IMAGE:7715793
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Gene:
sult1c2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975655 [+]
tttgttggcttcataccccaaagcaggaatcacatggatgcaggaggtggtggatatgatttaccaggaaggggacacaaacaagtgcctcagagccccc
acttatgacagacaccccttcctcgaagcagtgccccccaaaccggtgccatcaggtctgcagttagccgaagagatggaacccccccgggtccttaaaa
ctcaccttcccatacagctgatccctccatcattttggaagcagaactgtaaggtaatggtgggaaggcaagactgatgcacagaacgcctctccatggc
aaccgttgcccggtcctgatgctgtccctattaaaactttcattttaaaccccacgtaggtgatatacgttgcacggaatgcgaaggacacgctggtctc
atactttcacttccagaggatgaccaaaggcctccccgacccggggacctgggacgattactttatggcattcctgtctgggacacttccttggggcagt
tggtttgaccatgtaaacggctggtggaaggcaaaagacaagcatcgagtcctgtatgtattttatgaggacatgaagaaggtgagaaaccttgtttttc
ttcaactttgtagatatttatttagataagtggcttttcctttattcacccacccttagccattatgcaccttgccgaatagcttggagccatcatcatc
attaaacgatgtgggaagggggtcatgaagcatttgggggccttgtatttaagggtacggtgctgctgctgtatatgcgattcttcctgagattctctct
taccgcgcaggatctgaggcttgagattcagagggtcgagagtttcct
3' EST: CX975654 [+]agggcgagaggaagagatcaaatgcgaaaatgcagtaacttcgatatgaaaactcgttattatgggcccgggccctgcccatcagatctgggtgcagaaa
tccaggccagttccgtccattctccgtttatattcccagtcaaacaattcattctgggccaccaggaagtggttcttccagtccccgacaatccctttcc
gcatgaaaggggagacagattggtccatcacagtggtgggcatggtggtgtaattagccattgggttctccttcatggcctggaaggttgtgtgctggca
gatcttctccagaacctcttcagggagatccttatccaggaaactctcgaccctctgaatctcaagcctcagatcctgcgcggtaagagagaatctcagg
aagaatcgcatatacagcagcagcaccgtacccttaaatacaaggcccccaaatgcttcatgacccccttcccacatcgtttaatgatgatgatggctcc
aagctattcggcaaggtgcataatggctaagggtgggtgaataaaggaaaagccacttatctaaataaatatctacaaagttgaagaaaaacaaggtttc
tcaccttcttcatgtcctcataaaatacatacaggactcgatgcttgtcttttgccttccaccagccgtttacatggtcaaaccaactgccccaaggaag
tgtcccagacaggaatgccataaagtaatcgtcccaggtccccgggtcggggaggcctttggtcatcctctggaagtgaaagtatgagaccagcgtgtcc
ttcgcattccgtgcaacgtatatcacctacgtggggtttaaaatgaaagttttaatagggacagcatca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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