XB-CLONE-3549784
Clone Name: IMAGE:7715655
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Gene:
apoa4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975692 [+]
ccgatgtcagaacagaccaagcagtcagtgctttctgggactatgtctcccagctgcaaagcagcaccaaggatacggtggaccagatccagcagtcaga
tatcaccaaacaacttaatgttctcatacaggataaaatgaaaagtatgaacctttatgctggagaactacaaagccaagtaattccctttgcaaagcaa
atccatgaccaactaacacaagattcagagaaaattcgagagcagatacaacaggagctggagcagctcagggtgaagatttctccttacgcagatgaag
tacataaacaagtgaacaagaatttggaggagctgcagctcaaactgactccttatgctgaggaactgaaaagccaactagagaagaacactaacacaat
cagtcagcaacttaggtctgtagttcaagaaatggattctaagattagggacaatgcaggaaatttgcaagaatctttggcaccatattcagaaaaactc
caggcccaaatagatgagagtgtagaccaactaagaaaacaacttaacccttacactgatgaagtgagagataagattggccatcaagtacaaaagttac
gtgagagcctgagcccttatgcagaggagatgcaggagaatttagccaagcagatagagagcatggaattccaaatgagaaagagtgttgagcaagcaga
cacccaattaaaacagatcactgagcagttaaaagctcaactgagcccctatgcagatgatctgaaagagaaactccaggtaaatgctagggatgctaag
cagacactagagccatatctcaccgagctaaaccgcagatggagcagaagattgcagaatttaaagtt
3' EST: CX975691 [+]tgcatatcaaccacaacgtaaaacattacagggccttatgttgttactggggaggttcatcccagacataatgaccaagaaacaatggcagcagaattat
aaataacatggaacattttagggctgtaagagaaacataaagctattcaggcacaacaaatgacagacataacacaaagacaaatctgtgttttacaaaa
cttgctacagtgtgcatttacttcacttttacattttcttcttacttgatttgcagtaggctgacagcccaatctctgggtattttttttctttttatga
ttgatatggcatacaggtggtgcaagttgtaatggatactgtggtctgatgacaatttgggtgtatacgttgtttagttttcagtttcagttataccttt
atttatgaagtcgctaaacttatctcggatgtctttttctaagtattccatttgatcttgcacagtcactgtgtatttcccaaatttcagtttcatgtcc
tctaccctctgcactagtgccttgttgagtgcctctccatagggagtaatgctaactttaaattctgcaatcttctgctccatctgctggtttagctcgg
tgagatatggctctagtgtctgcttagcatccctagcatttacctggagtttctctttcagatcatctgcataggggctcagttgagcttttaactgctc
agtgatctgttttaattgggtgtctgcttgctcaacactctttctcatttgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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