XB-CLONE-3549755
Clone Name: IMAGE:7715801
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Gene:
ddx31
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975711 [+]
tctgattctaaatatcactcagtctcctgcccccaattccttccagaggggcaacagcaagttcaacaagccccacaagcacacagggagatgggcaaaa
tctaaatctgttaagagaaaatctcaagacgccagtgatgagccctccccaaagcgcccggtcccaccgactagccagaaacggctacgaaatgcccctg
atgaaaagccctataagaagcaagaagtccctgccgggaaggtccttgatggaaagaaggaggagaatccaaaaggacgacctgctgtgaagacctcttc
cctcttcaagaacaaccctgaggttccggatattgacagacctgcagtaaagcagttaaaagaaaaggtgttttctgccgactctttctctgatctgggc
cttcatcctcacctggtttcaacaattaacacagttatgaatatgacaaatacgacaagtgttcagaaacagaccatccccgccctccttgcaggccgag
atgtcctggtacgatcacagacaggttctggaaagacaatggcctatggagttccgcttgttcagtcgctccaaggggtgactcccaagatccagcgcag
tgatggtccatatgccctcgttttagtgcccactagagagcttgctctgcagagtttctccactatccagaagctactcaagcctttcacctggattgtt
ccgggagttctgatgggaggagagaaaaggaagtcggaaaaggccaggctccgtaagggaataaacattctcatttccacaccgggcaggctgctcgacc
acatccggtccaccaaaagtattcactttacccgggctcgctggctg
3' EST: CX975710 [+]cgctcctatagggcgagaggctttgcatttatacatgggggtaggagctgccatattggctgagatgctctctatagaacaacagagaggctctcagcta
cggcaagcagcatggcacctccagcacatcctctcattattactgcacccgtgcgtgacctggggtaggtgctggcagcacaaaggctatagctatggca
cccaaggtgttgctcctgcagttatgatgggttcgtcgtagaatattctgtgtttgtgttgttatacccccatcaccgtctgtattccacatgtctctcg
tggggcggtttcacgtctgggacccctcagtccccggcttggtggctttcttgggcttaggagccaagccactggcgtactcggagcgcagcaagtcgga
aaagcgctgcttggatctcttcttgtgcgtatcagccctcttcggtttgcctttgctttgcttactggggctccctgctaactgtcggctgaggctgtgc
ggggcgtctcgcaagccgaaactctttgccacgtggcccaggtgcaaggaacggatgtggaagatgtgtttgaggttcttggggtaggtggcataggagc
ggatgtaagactgcagcgctttctttgcccgcagggccgtatctgcgctggcatgaacataatcctcgaacttagtctgcaagacagtagctcgttccct
caccgtctgggggtcggcagcttccgatttcttgcctccagcacgcctgattttc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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