XB-CLONE-3549650
Clone Name: IMAGE:7715811
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Gene:
ddx21
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975784 [+]
ccgtgtgagagatcttgtccaaaactaccgtttggacctcactgttttaaagcatgtggtactagacgaagttgacatgatgtttgatatgggcttctcg
gaacaagtcgaggaaatattatctgttcgctacaaacctgatcctgaagaaaatccccagactcttcttttctctgcaacatgtccagactggatgtaca
atgtggctaaaaaatatatgaaaaaacagtttgaaaaaaattgacttggttggacatagaagtcagaaggctgccatcactgttgagcatttggccattg
agtgcaccaggtcccagaaggcagcggtccttggagatatagttcaggtctacagtggtagtcacggaaaatccatcatcttttgtgattcaaaattaga
agctcacgaattagccacaaactgtggctcattaaaacagtctgccaaaccattacatggggacctccagcaaaaggaaagggaagtcatcttgaagggt
ttcaggcagggatcatttgaagttcttgttgcgaccaatgtagctgctcgtggattggatatcccagaagttgaccttgtagtattgtactctgcaccaa
aggaagcagatgcttatgttcatcggtcagggcgcacaggaagagccggacgcacaggaatctgcatatccctttatgaaccaagagaaaggcattatct
tcggaatgtggagaggagcacgggaatcacattcaaacgtgttggaataccttccttattgaatgttgccaaatcatccagtgctgatgcaataaagtcc
ttggattcagttccagctgatgtgattgagcatttaaagaatacgcccagagctgattg
3' EST: CX975783 [+]gttttaacagatagattttaataagggctaagcacacagttttatattgcataaaaatatgtctcaagttactttaggaaggaatatttatgaacatggc
agtatattaaaaaaagttattgtgacatgggatgttgggatgtttggagtcactaacggccccttctaaaccctccacttctatttctgcctcttcctcc
tcctcctcttcctcctctggaattatttcttctatcaaaaggtctcctccctcttcctccaaaacctccattccttggaccatcaaagtttctctcagat
tcttgaatcgcgggtaattcagttgcaactgtaaactgccaccgccttgtatctttccagccctcctgcattgactgcaggttttcagaacggacatcaa
aacacacacccatggaatccttcaggagacacattctatgaattttagaatcaatatcttcacccagttgttctttaattgatcgccatgcatagcttaa
gttgtgaatttgcactgagctcttcaacgttattgtcacataacccgcttccatgttgagcaaagagcgttgtttaatggacgtcgccccagaaatatga
gctaaagctgcagctagggcagttaatgcacccttcttctcaatcagctcctgggcgtattctttaaaatgctcaatcacatcagctggaactgaatcca
aggactttattgcatcagcactggatgatttggcaacattcaataaggaaggtattccaacacgtttgaatgtgattcccgtgctcctctccacattccg
aagataatgcctttctcttggttcataaagggatatgcagattcctgtgcgtccgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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