XB-CLONE-3549610
Clone Name: IMAGE:7715815
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Gene:
gtf3c4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975813 [+]
ccaaagtgagacccctccgtgatccttgggaccagggaaagggataacagtaatggaggaaacctctcattattgctattaaagtgcaatagttaaggaa
gcgctgtggcagctccggtaccagacagtccttgttacctcagtgcagtatgagatccatacagtgtaggcgatgctgcattacaaagcaaccttcctgc
agaaatgatgaggaggaggaggcggtgtttccatggaccaatcattgtgcacgtggcgctgaggtgacccaatcagagctctagaatacaggggagtcac
acagtatgcatggataattcaacagcttcttcatccaatctggatctttgggtgccggatgtctggcgatgctgtcgtgcagcaagcaccgtcggtacag
gaggctctgacaggcctggtatgtaaggaaacatctgggccatacatgcccattggggcaaagagcctgctttgggtcattaaagggcaacacttcttta
cacagaatgcagtgctctgggaacttctgcttgttcatcttcttctctaggtgcccgatgatcacctgaactgctcgatcatccgagtctttagaagtca
gaaagtcacagatacccctagtgggaacgctggtattctgcgcaatccacgtgtgcagataaacttcccccaacacttgtttcatgtgttcccgtactaa
gtgccactccgccgagccgatcttctcctggatctccagcaagccgtcttctgccgctccgccgtccgcctctctggtaacgtcagcagggctattctcg
ctg
3' EST: CX975812 [+]cgctgacagtcagccagtcctttatgctggatcgggtgtttaaccctgaagggaagttcttaccgtctttccaagggtttaaatacgccagctggtctcc
tattggttgcgacgccaatggccgatgccttgtggcagccctcaccttggacaacaggctgaccattcacgccaatattaaccggctgcactggagacag
ctagttgatttaaccgaactgtatgggcagcacctgtttgagagcaactacagagtaagcggggacgagaccccccacgcgggcctggatgactttactg
agttcaagaggcggcacagcatgcagacccccgtgagaatggaatggtcggggatctgtatcagtcaacagatcacggagaataacgagtgcgtggatca
gagcacggtactcctggctgtactcttcgagaatggagacgttgcagtgtggcagctccggctgccctttctgggcaaggaatccatcacttcttgcaac
accattgtatcgggaatatcttcccccagtgttctggcgtggtgggaatacgagcacagcaatcggaaaatgagcggcctcattatcgggagccgtttgg
gccccattaaaatcatcccagtgaacttaaaggcagtgaagggttacttcactctacggcagcctgtgatcctttggcaagaagccgacttgctgcccgt
acacggcattaaatgcataggactttatcatccgtaccagaactgccgctgcagccttgtcgtcgctgcccggnggtgctatttattttggtgtttgctg
ctcatatccaaagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: