XB-CLONE-3549537
Clone Name: IMAGE:7715965
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Gene:
acta2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975867 [+]
gggccggcttcgtcgtattcgggtttgctgatccacatctgctggaaggtagacagggaggcgaggatggatccaccaatccagacggagtacttgcgct
caggtggggcaatgatcttgattttcattgtgctgggggccaaagctgtgatctccttctgcattctgtcagcgatgccggggtacatggtggtgccacc
ggacagtacgttgttggcatacaggtccttcctgatgtctatgtcgcatttcatgatgctgttgtatgtggtttcatggatgccagcagattccatacca
atgaaggatggctgaaacagggtctcggggcagcggaaacgctcatttccaattgtgatgacctgaccgtcgggaagttcgtagctcttttccagagagg
aggaagaggcggcggtcgccatttcattctcaaagtcgagggctacgtagcacagcttctccttgatgtctcgcacgatttcacgctcagcagtagttac
gaaggaatagccgcgttcagtcaagatcttcatgaggtagtctgttaggtcacggccagccaggtcaagacgcatgattgcatgagggagagcataccct
tcatagattggtacgttgtgggtgacaccatcaccagagtcaagtacgataccagtagtacggccagaggcatagagggatagcactgcctgaatagcaa
cgtacattgcggggacgttaaatgtctcgaacattatctgggtcattttttctctgtttgctttgngattcagtggagcctcagtgagcagggttgggtg
ttcctc
3' EST: CX975866 [+]cgacaacggctctgggctctgcaaggctgggtttgctggggacgatgctccaagggccgtattcccttccatcgtgggtcgccccaggcaccagggtgtg
atggttggtatgggtcagaaggacagctatgttggtgatgaagctcagagcaagagaggtatcctgacactgaagtacccaattgaacatggcatcatta
caaactgggatgatatggagaagatctggcaccacagcttctacaatgaattacgagtggcccccgaggaacacccaaccctgctcactgaggctccact
gaatcccaaagcaaacagagaaaaaatgacccagataatgttcgagacatttaacgtccccgcaatgtacgttgctattcaggcagtgctatccctctat
gcctctggccgtactactggtatcgtacttgactctggtgatggtgtcacccacaacgtaccaatctatgaagggtatgctctccctcatgcaatcatgc
gtcttgacctggctggccgtgacctaacagactacctcatgaagatcttgactgaacgcggctattccttcgtaactactgctgagcgtgaaatcgtgcg
agacatcaaggagaagctgtgctacgtagccctcgactttgagaatgaaatggcgaccgccgcctcttcctcctctctggaaaagagctacgaacttccc
gacggtcaggtcatcacaattggaaatgagcgtttccgctgccccgagaccctgtttcagccatccttcattggtatggaatctgctggcatccatgaaa
cacatacaacagcatcatgaaatgcgaca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: