XB-CLONE-3549495
Clone Name: IMAGE:7715635
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Gene:
snd1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975895 [+]
ctttatcggcagaataatggcatcttctggggctcaaactggacccgtggcacccgcactgcagagggggatcgtcaagatggtgctctcaggatgtgct
ataattgtacggggccaaccacgtggagggccacctccagagaggcagatcaatctcagtaatatccgtacaggagctcttgcccgccgggctgctgcat
ctcagcaagatgcaaaggacacccccgatgagccttgggcttttcctgcccgggagttcctgaggaaaaagctgattggaaaagaggtttgctttactgt
ggatttcaagtcttctcaggggagagaatatggcatggtttacctgggaaaagataccagtggagagaacatttctgagtcacttgttgctgaaggactg
gcctcacgacgtgaaggagtgcgtgcaaacactcctgaacaaagccgtctggcagaagttgaagagcaagcaagatcagcaaagaagggagtttggagtg
aaggtacaggatctcaaactgtacgggacctcaaatacacaatagaaaatccaagacattttgtggattccatgcaccagaagcctgttaatgccataat
tgagcatgtgagagatggcagcgtggttcgagctctgctgcttcctgattgctatcttgtaacagtcatgctgtctggaataaagtgccccacct
3' EST: CX975894 [+]gaccctggacaaagggaggaaaattccagatgatatttgataataataagacagataaaatcattacaagtaaccgttaaaaggacaatttcaggacgca
ggactgaatcacacgtaactcatacaaaactactttcagcccataggtatgcttctgcctttagaattgtagaatacatttttttctgggtgcagacaga
tggaagtgggaaagacgtaaatgttaaaatagttgtatttatattgtccaggattaccccctgtgtgcagcctgcagggtgtcaactgcgagttccataa
aaacctccccatgcctaccttttatgctcaggcaggacaggggtaggttaaggagggtctgcaggcaacaagagcaaaatggctgcgtaagatggctggg
tctcatctttggaatggcgtcccttaacggctatagccaaattcatccgcatcatctgctcggaaatctccgtaacgccacagattcaaccgagcagcct
tggcagactcttgagcagacacgtattccgcaatcactttctgaaactgcttttccttgcgaacttccaccatcacaagaccttctttcaccaagcctaa
gcccacatcttccttggagtctgcaaactgcagcgtaacatgtgggcaaccagctccactgtgctccacattcagcaagcactgtgtattctgaatgtca
cgaacaacgttgtccaccacatcagcccgagcatcttcatcagcgggcacttggatgaaagcaaagcaatactctatagcctgtgctggaagggtgcgtg
tactgaaagactgtgggagaggaccca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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