XB-CLONE-3549388
Clone Name: IMAGE:7715646
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Gene:
apob
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975972 [+]
tactgatctaagcataaactggaaggacgatatcaactttaatggtttaaggattcctggacataacattgatatagacatcaaaagtccaacctttaca
gatgtccaaatacgtagtcaacaggaaaataacaaaataacttcatccgtgtcatcttcatctgccggcactctggggcttgtacttgagaaggaatcct
cagccctgaaagggaaactctttagtcggtccttgtcaacaccaggaaaagacacattaataatgaaaggtgaggtgtcccttaaaaatcctgaacgcat
tcagtacaaaatcaactggagagaagaagcagctatggatgtacttaatggtttgaaagaaaggttaccaaagatttccaactccatccataattgtgtc
aataaataccacaaggagcattttggaatggaaatcagtgaagcaggtctaaagatgaaggaaaatctccaggataaaattgcatcaacctacagatcta
ctgccaatgagatcaacaacatagaatatcagttgcagtcaacagccagaggagccagtgacaaatataatgaaatcgtcaacagtgcccaaaaatggta
tcaagatccaaaggatatgtcaatgttcttgaatgatggtgaatataaagacaaagccattgaattagtacgcgcctaccaaaataagatgaaagacttg
attgatgcagctattaaatttgtgaaaaacacaagattccagttgccaggacaaagcagaaagtatacaggtgaagagctatttaaccttggtatgaaaa
atgtagcgagcaacattgatgcgtttt
3' EST: CX975971 [+]taatgatgattttgcataatgcaaacaatgaaatgataatagttaaaaataatgccatcctataagaaacaatctttttattagcttacaataacgaaaa
caaatttttgaattgttcacacaaaaacacacatttgagggacgtgtaaaccggttgttgctactgtacttgcaaacaacacaaaagcctacgtttcagc
tatgccataatatacagcctgctcagtttttttgccagttccgaatgttctcaatgatctgctctgcagttagtttcccttcttgaagttgtctctcgat
aaactgctgcagctcttcccatttctgagaacctttgtcgatgccatccagtaccatggcgcgcgcaatttctgctcgtttgcttataatgtcctctctt
aaatgcgggatttcatcgaaggatttccagtcaaagggatgagggacttcaaccttaagttctcctttacgtgtggatatataggtcttcatttcttcag
tggccgaattctgaatgctttggagaagctggttgacatactctatgaaagagttgtagttttggatgataatgtcaatgagtcggcttgcctcggcaat
aagcttctcgtatgaatttacaaacaggttagatgtagcttgaagtttttcattaagaatcttattggcattttgtgaaagttgcctggcagttgcagac
cacttagttactctatcttcaagatctattgaagccctccttatgagttctttgccttgtccattagcattggtcagcaaatcgttagcatgttggtaat
atttttttacaaagtcctttgtttgtt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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