XB-CLONE-3549286
Clone Name: IMAGE:7715942
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Gene:
calr
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976045 [+]
gctgcttgcggctctgtgcatcgcagatcctgctgtttacttaaaggaagagttcgctgatggagatgggtggtcgcagcgctgggtagaatccaagcac
aaatctgactatggaaagttcaagctaagcgccgggaagttctacggagacgcagagaaggacaaaggtctccagacaagccaggatgcccgcttctacg
ccctgtctacccgctttgattccttctccaacaaagataagacccttgtcatccagttttccgtaaagcacgagcagaacatagactgcggcggcggata
cgtgaagcttttccctgcggagctggagcagtccgaaatgcacggcgaatcagagtacaacatcatgttcggccctgacatttgcggcccccccacaaag
aaggtgcatgttatcttccagtacaagaagaagaaccttcagatcaacaaggatatccgctgcagggacgattctttcacccacttgtacaccttgatcg
tgcgcccagacaacacctatgaagtaaagattgacaacagcaaggtggaatccggaagcctggaggaggactgggacttcctgccagccaagaagattaa
ggacccagaggccaagaagcccgatgactgggacgagcgccccaagatcgatgacccagaagacaagaagccagaggattgggataaggctgaacacatt
ccagaccctgatgccgtgaagcctgaagactgggatgaggagatggatggcgagtgggagcccccagttatacagaacccagactacaagggagaatggg
aaccccgtcagatcgataacccaga
3' EST: CX976044 [+]tccaaaacaaaacccatcgaggctccagggatgagccctgccacaatataaattagaggcgtaaatcaaactggggattggaggacagtggtctttataa
aaggaatgaatccccctggacactaaaaagggaatacactggcaggttggttattggacaacacttgtggaatgcagatggaggggccgcagccaatctg
tataataccctgcaggtttagcaaagtcacctaacactataaggcattgctgtagagctgggagtggtagttcagcaggccgcaggctgggatttactgc
tgtaagcaatcactaatgataatagcaatagccctgtttcacccatgcacaattgtgttaccgccgcagggtcactagcagccaacccccctattctaat
ccaggcccccggtataggtgcagagaagtatggctgtgtgtatgctaagcttgagttctatgctagccagccgagggccacactggccagcactgtacgg
cttattaaaaccaccacttccccaccaggcaatagccccacatagagaacactagctgggcttttaacacgcaagcggccatgttatcaatgcttggctt
ttaaaatatgaacactgatttgattcgatactgcattacgggaggaaactcaaatacaactgtcgccctgcctaaggaacccttgttgagctgggtattc
ccaggcagaacgtagacaaatctggcatgattttgattggccagaccatatctgtgggcgtggttatttcctaattgaggaaggcaaagagttactgnca
tggagggactgaagggaagggaccag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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