XB-CLONE-3549276
Clone Name: IMAGE:7715800
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Gene:
add1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976053 [+]
cgtaccccttccttcctcaaaaagaacaaaaagaagagcgagtcgtgagtcgtaactgcaggcgcactgtcatgtttctaagggtctgctcatagccgct
agaatgtaccccattgtatcgtctagtctcattatattaaggaatgccactgaaccccctcgttagttagcgcttcattctcatggttaatccgcgaaaa
ctcactcgcatttaaggtgctaaccaacgccgcgttcagctgataatctggggtcacctaactgcacagtgtaagcctatagtctcctcttgttttattg
ctgtacgtgactgctcagtaacccccacacctgtcccccccatattctctcacccttccttgtaaatacacttctctgctttgccttgtaaggaccacga
tgcccctctgcgccccaagatatgtacccaatggtaaattcctccgtgccctatgcaccccatggtaaattcctccgtgccctatgcaccccatggtaaa
ttccttagcaccctatggtaaattcctcagtgccctatggtgcatttatgcagggtatgatagcttctaactggccaatcccagttgggtgcagcactgg
tgtcttctgcccccgccttagtggccgacaccattgctgcagcacagctctgggggaggagcttaaatgtgtaattcccgttaagcaaagatcattttaa
tggtatattccagaattctactctgtatttttggtaagggaacaaatatgttgtgtgctgaatgttgcttgctattggtgcccgcactgaattctggaag
gctttggggat
3' EST: CX976052 [+]agggcgagaggcttaattgaaatcaaacggaaaatcgttcagaatattttccataaaagttcaatggttgcctcttctatccccaaagccttccagaatt
cagtgcgggcaccaatagcaagcaacattcagcacacaacatatttgttcccttaccaaaaatacagagtagaattctggaatataccattaaaatgatc
tttgcttaacgggaattacacatttaagctcctcccccagagctgtgctgcagcaatggtgtcggccactaaggcgggggcagaagacaccagtgctgca
cccaactgggattggccagttagaagctatcataccctgcataaatgcaccatagggcactgaggaatttaccatagggtgctaaggaatttaccatggg
gtgcatagggcacggaggaatttaccatggggtgcatagggcacggaggaatttaccattgggtacatatcttggggcgcagaggggcatcgtggtcctt
acaaggcaaagcagagaagtgtatttacaaggaagggtgagagaatatgggggggacaggtgtgggggttactgagcagtcacgtacagcaataaaacaa
gaggagactataggcttacactgtgcagttaggtgaccccagattatcagctgaacgcggcgttggttagcaccttaaatgcgagtgagttttcgcggat
taaccatgagaatgaagcgctaactaacgagggggttcagtggcattccttaatataatgagactagacgatacaatggggtacattctagcggctatga
gcagacccttagaaacatgacagtgcgcctgcagttacgactcacgactcgctcttctt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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