XB-CLONE-3549187
Clone Name: IMAGE:7715762
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Gene:
aspg
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976116 [+]
ttaaaacattacattaccaaagcaggaggcaggaaacctttgctatgtgtacaattgcaaactgtaaaagcaattaaatttcctgctggaagaatgacaa
ataatgttacagtcctaaaacctgtttaacatacattgatcttctgaacctttaataatcatttttacaccaataatactgtgctattctgggtcaatca
tgttcagaaaataaagtgcagcaagtctgggtaaaacaactgtacccaaggtgcagcagctaaatcttttgccatttccagcaacactagagtgcagttt
attgccctagcatggctccctataaatgccatgctccctatgcgtcagagaaacactcattatccagagatcttctgagccagcagcctattcgtgggaa
gagacaaacactacattcataaacatgcaaccacgaagacgaaatgtttcaacataaaatgcagggagtcacacccattgtctgcactccccgcagaata
atacacaacttgtttcatgtgctgatactaacactaatatgtgtgcatttgttaaaaaaaacaaactgcagattcacacaaaatacttatataacaatca
taaactatatttaagttttctcttattatgggggaatgtaataagtcgtaaattttaaaaaatattgttcattatactgttattgcattgctaataattt
ttcagttaccgacttttattacattaactccatagtctattacctttctaaaatagtttgcgcatgccatttaattatggtgacagaatgcgctccaggc
accacaggatatggtccctaactgatctg
3' EST: CX976115 [+]tttgcctcactacctggctaatgaatgacaatctgggcactcctctatgtatatataaagataaaagaagttgtaggggataatacactgaacaatttgt
tgaggaaaattttacctgttttagaaagcacacaaatcagtcagtcatgcacaatacatggttgtaaggggtccagtacctggggtaaaggacaatcttc
ccatcaaatatataggcctttcttttctatttgaaagatgccctggactccattctttagaggtctaatgggggcccatgatccacattattaaaatcca
agagacacatgtgcaaattagtagttgtcacggctactaaatgctaggaaataccctgccatggacaatactgagaactgcttctgctaaaagagccaag
ctgcatcatgtagtctctagcagcattgtgagcacaatgtaaggcaaccaggcaaaatcatgaagccccaaacccatggatagcaaatagactaccctgc
ccttcacgaaaaccaaatcctaaaccacctttcccgaaaagatttgtctggaatcaaacaaaccctgttttattcaataataattgggatcagcaggaga
gttggaacttatatcacaccatcggtgaatagggttgcattctggctgatattttacccaccatgctggtgagaatataattgtttggattcagatcagt
tagggaccatatcctgtggtgcctggagcgcattctgtcaccataattaaatggcatgcgcaaactattttagaaaggtaatagactatggagttaatgt
aataaaagtcggtaact
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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