XB-CLONE-3549122
Clone Name: IMAGE:7715768
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Gene:
glul
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976162 [+]
cgtctcccacagttcgagactcaacaagggggtgagggaacagtacatgaaactgccccaaggagggaaggtccaggtcacctacgtctggatcgacggc
accggggagggagtgaggtgcaagacccggactctggatcaggaacccaaaaccatagacgaaatcccagaatggaacttcgatggatccagtacgtacc
aagcagaaggttcaaacagtgacatgtatctcgtcccagtccagatgttcagagaccccttctgcctggaccccaataagctggttatgtgtgaagtctt
aaaatacaaccgcaagtctgcagagaccaacctccgacacacgtgtaagaagatcatggaaatggtgactgaccatcgcccatggtttggaatggaacag
gaatacaccctgctgggcattaacgggcacccgtatggttggccagagaacggcttccctgggccacaaggtccctattactgcggcgtgggggcggata
aggtgtacgggcgggacatcgtggaggcgcactacaaggcgtgtctatacgccggcatcaagatctgtggcaccaacgcggaagtcatgccctcccagtg
ggagttccaggtgggaccgtgcgaaggcatcgacatgggggaccacctatggatggccaggttcatcctccaccgggtgtgcgaggacttcggggtggtg
gccacgctggaccccaagcccatgaccggaaactggaacggcgctggttgccataccaactacagtacagagagcatgagggtcg
3' EST: CX976161 [+]agggcgagaggctgagtacagtcgatatcgtgggtataaagacagttgtataaagcccgtagctccagaggagccaattaacaatcccgccctccccaaa
gggagaaactgaaggaatcagtcggcaccagccaatcagattccagcagggtcactcgctttgttcttatcctatagatctaaaataatcagcagccccc
cccaaccttacagacaatatggtagaaccatctatacaccttagaagaattcaagtagtgtcgggagtctaacgggttaaaaaaacatggcggcgtctcg
ggagatgccatagtgattgaccaggagaagccagctcccagctcaggtctaatagtctttggtctcgctgccggtctcgttcaagatggtcgttcggacc
agcgcctcggtaacggcgtaggggtcgcagtttgctgccggccggcggtcctcgaagtaaccgtagccttcctgccccacctggcgggggatccggatgc
tggcaccccggttggcgactccggccgagaactcgtggatactggacgtctcgtgctgcccggtgagtctccgggagttgtccttcccgccccgggggtc
atacacgcagatgtggtagttgtgcctcttcccgagcttctcgatggcatcttctatgtgtttgagtcccccttcgaccctcatgctctctgtactgtag
ttggtatggcaaccagcgccgttccagtttccggtcatgggcttggggtccagcgtggccaccaccccgaagtcctcgcacacccggtggaggatgaacc
tggccatccataggtggtcccccatgtcgatgccttcgcacggtcccacctggaactcccactgggagggcatgacttccgccgtg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: