XB-CLONE-3548996
Clone Name: IMAGE:7715780
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Gene:
stxbp3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976248 [+]
caactttgtaagcaaatatcaaaataactgtctctttatgttgtaattgtgtttgcatcctactgctctagttacatgaaataagtcaggattttaccat
ttcctgggacttgtttctttctctctggggagatattttttcagtatttatgtctctgttatgccttgcagtacaacagttgtgccatttattgcttctc
tggttgccagatatattaactttttgttaacaaaactgaaaaggagggttaacaggagaaatgatttatgtgtccggctgacaaacagtataaggctggg
tttacctcagtggtaaaatgtataacaaagggttgctgtgggcccttgggcttatcattcagccactagaaggtagttggtatttcctaaataaagttgg
cacttatctggctttgcagataatggatctttccattatttgggtcaccataccttgaggcctgctaaaaaatagtttaaccattaaataaacccagtag
aattttagcttagttcccatcaagtacacagtactatttaatacagaagaaagggatctcggtaaaatacatttgaattatttgcttataatgtgctcta
tgggagatgctcttcctgtaattcgtaggtttctggataatgggtttccagataactgatcccatacctgttatggtataaaggcaaattagcttaacga
ggagccatcttagggctttggcacacagattagtaatctccccaaaatgccatcccaccggtgagaatgtaaatcgccggtgggaaatcacgggaagttt
ctctctaggcaacttgcgtgatttc
3' EST: CX976247 [+]ctcctatagggcgagnaggctgggaaaaaaaataaataaaacctacatttattctttaaatcttattttccaacatacaagtcttttgtgacctatggta
ttagaaatgccaaaatggctattggtggatcccttctaatcctctctaatacaagcctactgtaagatacgtggctagtgatacgtggtccagaattaaa
ggaggttgattaccttaaaattaacttttagtattatgtaaagcttgatattctgagacaatttgcaattggtctttaccttttattttctgtggttctt
gactaattatttagcttttcattcagcagctctccagtttggaatttcagcaggtatttggttgctagggttaaaattatccaagcaaccaggcagtggt
ttaaatgagaaactggaatatgaataggagagggtctccttttgagatctagtcggaagaaaaaggcaaatatttctaacactataagtaatataaaatt
aagaccatctgaatttgccaagaatcagccattctataacctactaagagccaactttaaggtgaaccaccactttaaacggagtcagtattcagcctct
tgttagtttgctagaatacaatttctaaaataatgctgttcagttgagatttctctctctccccatcgggtaaaggaaagacattttatatattaaaaat
aaaaaacgaactgagacagtgcatatgttaccattataaagtcagccgtaaaactgcattaatctcagtgaagcaaatgccaacattctctgtacaagtt
ctattttcagtgctttgcagagaaattcatttggttaaggattatntatatattanag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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