XB-CLONE-3548987
Clone Name: IMAGE:7715924
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Gene:
fsd1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976254 [+]
caaaaatgacaggggatttacaaagctttgggctggaattttaggaaacactctgtgcttctactccaatcacaaggatttgcggagcattgacaccatc
agtttggaaaattatgtatctctgaaggagcctggtggtaatttgtcatcagaaaatcaattttccttgtgcatgaaagggcgagaaatacagctaaagg
cagagtcattagagagccgggaaatgtggcgtgcatatatcatcaccatagcagagttgaggatccccccatctctaagcctgcttccagggcctcgtct
tgctttaaaggaagctttggagaaagaaatagagagaaaaaagaaaaatgaggcagaagaaaatgaaactcccagctgtttctataatgtgtcccggatg
gaagcagagaatttactgatgcaaaaccgtgaatgtggcaacctattattgcgacctgggggaaaccagaagacactgtctgtaagcacctgtcagaaat
ttcacagcaaggacgtagttaagcattaccgtgtaatgcaggaacaagagggatatgttattcaggtggaacccaaagttgtttgctcctcgctagcaaa
tgttatcagccactttgtgaatagttcgaacaaaaccctgaaaccctttgtaaagatctgtgaatatgagaataaaattgcaatttatgaacagaacaat
gaagatggagtggttacagtacgatatgcgcaggactccccaggaagcacaaaaacctccccacttcatactgcacctcctccccttccacgggatcctc
caccagaagatgattatgagaaccca
3' EST: CX976253 [+]ttattaggagcaactgattaatatttacagttaggagaaactccttttgaagttatatgacaaacacattgaccagaaatgtaaccataaaatatcagat
ctaaatcttaacattaaactcatcctgaacacaaagactaggcaagctgtaccttaatagtatgatggctttagtacaatatttgtattgttgctaatta
ccttctgtcaaccaacaggtatggtgtggaagtttctcagtgtatactggactccaggttcctagctgtgtgaaaagcctccagaaaaggaatagtgcca
ccagcagctcaagcaccagcctcacttgatttcatctgatctacccacctccttcatttccccacctagatctctccttttctctccctttacaatgcat
taaagcttaaagtccacccagtattttaggggacctattaaaaaaaacttgaacataatttagggttctacaacaaatattccatgatatcctctgcacc
tgtaagagatggatggacagtacgctaagtgtggcattctgctgattatgccttaaataaatataagttacatgtagtatcgcgatactgtcagcttgtt
tgtatgcataagctctatgtgtagcttctaaacatctataagctcatccccaagaactttctccatcttaggctgtttctccacctgctttttatttgtt
gtcatcagctcctttctgcatcagccatgtgtggaattagtctcagagccgaactatcttcactgcacatctgtaaatataatgcattcactgcactggt
ggtgcaagccttccatgggactggcagtgtcccta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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