XB-CLONE-3548844
Clone Name: IMAGE:7716129
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Gene:
atp1b1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976351 [+]
tcggctttcccatacccgcatacagtaaccagcgtgacagtggccagccagtcccattgttagataacaggagaattgtagggacaaggaagaagaagca
gcccagtcacgcttcccatcagcgcccagtgacatctcaatagccggtaccgcaaaataacacagaacccttaactgaagtaggacaatatggccaggga
caaagccaaggaaaccgacggcggctggaggaaatttatctggaatcccgataagaaggaatttcttggcagaacaggtggcagctggtttaaaattctt
ctgttctatctcatcttctatgggtgccttgctggcatatttattgggaccattcaagtactcctgttaaccatcagtgaatatgaaccaaagtatcaag
atcgtgttgcacctccaggactgacacaagtacccaaagccgtcaaaactgaaatcaacttttcaccaaacgatcctgacagctacaatgattacgtgca
aagcatggaaaagtttatttcgaagtacagcaacgagaaccaagtatccgataagtttgaagattgtggcactatgcctgggcagtaccgtgagaggggg
ggtcttaacaaggatgggggacagaaaaaatcctgtgtgttcagacgtcaatggttgcagaactgctctggcatagatgatcaaacatttggatttgcag
agggcaagccatgcgtcatcgtgaagctcaaccgaattgtggcatttaagccagtgcctccacagaataacagtcttccaccagaaatgacagcgaacta
caacccatacgtcatccctatccactgtca
3' EST: CX976350 [+]ttaacttgactgaagttccaacaagagcacaggactcctggcaattattacagatgtatgaacaatgtgtgcacagtagacatttaaatacatcctatgg
gatactgcattgccatgaacgttgttttaaaataaagatttatttttcaacaggttactttggtttgtttcatacattttctttttcttgaagaaaaata
aaaattgtacaactgcataaatataaaattcttccaccatgaaaatggttaaaacatttgtaaagacaaagcaccaaactgtgatgctttcagaaaaaac
aaaaaaacaaaaacataaaataaaaatatttatatatatataactcaaaaaccattagccctatttctgacaaaaagtgacagtcggacccggggcttca
cacaccttcataatgggcttaagcatttaaaggggaaagtttctataaaaagcattaaataaaaagcaccgcacaacccatcacctcttgcatttcagtg
aaactaagaagagaaagacaaaaacagaagctcttttacagataaaacaaatcaatgccttatttaaaggacttgtggagtgctattaaatattattggt
tggccaccacttccgtttaaattaaaaaaagaaaaaacaaaacaagaaaagtagcaataaaattaccatgccagttttatccccattacagactatttac
accttggactacaaacttgctcacaaagcagaaacaaaacaaaacatggcttggccgagactgtagaatctgcacttatcctactgtacaaacacactaa
aataaaaaaaagaaaagcagctatagtttgtggccagaaacactgcttttactacattttgatgatggaataacaatttacag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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