XB-CLONE-3548813
Clone Name: IMAGE:7716323
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Gene:
ewsr1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976373 [+]
ctttaccattgcctttggacctagaagatgagctagaaagcagtaccatatatgtacagggactgaatgacaatgtgactgtagaagaaatagtggattt
ctttaaacactgtggtgatgtcaagataaacaagcggacaggcgaaccgcttgtaaacctctttatggacaaggaaactgggaagcccaaaggagatggg
actgtgtcctttgaagacccaccttcagctaagactgctatagagctttgtgatgggaaggatcttaatgggaacaaggtgaaagtgtcattggctcgca
aaaagtctttgctaggtagcatgagaggggggtcacttttacgtgacaacagaggacagccgccacctctccgtggaggtcccatgggtcgcttgggtgg
ccgaggaggggaacgtgggggattcatgccaagagggccacgtgggccaagaggtagtccagttagcggtaatgtccagcacagggctggtgactggcag
tgccccaaccccggatgtggaaaccagaattttgcttggagaacagaatgcaaccagtgcaaggctccaaagcctgatggccctttccctcctttcccac
cccaaggaggagaacgtggaagaggtggtccaggcatgagaggaggcagaggcctaatggaccgtggtgtgcctggtatgtttagaggtggtcgtggagg
agatagaggaggcttcagagggcgtggtatggacagaggatttggaggaggaaggagaggaggacctccaggtccccctggtccactcatgggacagctt
ggt
3' EST: CX976372 [+]aaaattacatttattgaggagttccaatttgtaaaatagtggttcaacaagattttattacagtattatggggtggggtccaacaaattaacacaagcca
gtatattaaaacaaaaataaaataaatccccattttttacagaatggccacaaaataatatactcaccaatagcaagcccacccctccccaaaaaacaaa
caaaagttaaacacaaatccacagtcgcataagagggtattattagccatttgaagatccaagacaggggccaaacagattcacctgctaaaaataaaaa
cagttttgtatggtctatcttctctagccatagattctgtcaagataatgcacatgctttcattcaaagacttcaaaaccatcaaccttcacatgcaaag
aagctaaaaattatgtttgctctcaaaaggaaggtaaaaaaaacaaaagcaaaacaaaaagacagcaatactaccattaaatttccattttaatatctcc
acaaattgtgaatactaaagttacagacaaagaataatcataatgtgacagtgaagggaggatcttcatgtcagcattataaatatggaattataaattt
aaaacatttctggtttaaaaaataaatctggtagtaaaggtggctccgaaactagtagggtctctctcttcgttcctggcgatgctcactcttatccatt
ttgctggggccacctcgtcttccaccaccaattcttccaccaagctgttccatgagtggaccagggggacctggaggtcctcctctccttcctcctccaa
atcctctgtccataccacgccctctgaagcctcctctatctcctccacgaccacctctaaacataccaggcacaccacggtcca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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