XB-CLONE-3548687
Clone Name: IMAGE:7716003
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Gene:
serinc3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976464 [+]
cctagctacacnctttccccacacatgaccactattaataaagacaggacaaagactggtcccagtctacgcttgggtgttagtagccaagaatactgca
atacactgcattacaaaacaaattcccctcaaggtttatcatccatcaatactgagtgtagcaggacaggaacgtaaaaatatataaacacaaatgtagg
aaaacaaaagcaatgacattaatttgacttaaagaaaagcagtgcatctgaacaaggggcatataaaatatccaacagtgatttcttactagtatgaaat
agacttaaatactctagatcagctcagctgtggcaaataaaaaggcagcaatgtatatatattatcaagctttaaagacaggaagtcaaaatctgtttaa
agtaagaaaacagccaacagttttacattggctcgattaaaggttttcgattatagtcaaatagtatttgagcagaatttgatgttcttggaatgaggtg
gttagctgaagtcacgattggagagaacaattggagctatcagcgtccagaagtagagaagcaggcatacccagctggagctgatcttaaaccacacggc
gggccatgtacttgtcatggttttggtgtctgcagttgggctgtaccaattagtcagagtcatcataatgtacagagaggagaggcagagcatgaaaagg
aagagggaatagttgtactggacgccatccttctcattgtctataacgcgtttcacttctccatcttctgcatcgctggatcctgttgtgtcatc
3' EST: CX976463 [+]gagaggcttggatacatgcctatgcagcagtgcgacgtgcctgctgtgccgttgctgcccgaataccggcaactcgacagtgacccgtattgtctatgcg
tttctgatgctgcttggggccatcctttcttgcataatgctctcaccaggcatatcggaccagttaaagaaggttcctggattttgtgaagatggctttg
ggacacaattaccacacgttgatggatacgtgaactgtaacgtgctggttggatacaaggccgtctatcgtgtcagtttcgctatgaccctgtttttcct
cgccatgagcatatttatgttaggagtcaagacaagcaaagatccacgtgctgcgatacacaatgggttttggttctttaaagtattggctctggctgga
attatggttggtgctttctatatcccagaaggacattttacatccgcctggttctggattggcgtttgtggggcctgttgtttcatcatcttccagttaa
tcctgctcgtggattttgcccattcacttaacgagctgggtgaatcgcatggaggacggaaactccaagtgttggtatgcagtgttactatcctttacca
ttttatgttattctttatctataatcggcgttgtcctcctctacgtatactacacgaagtccgacggttgcaccgagaacaaattcttcatcagtttcaa
catgatcctgtgtgtgatcgtatctgtcatttccatccttccaaaagtgcaggagcatcagccacggtctggtcttctccagtcctcggtgattacttta
tatacaatgtatctgacttggtcttcaatgtccaacgaacccgatcgatcttgcaacccaagcttgctgagcatcatatcgacaataaactcgccagcga
tcatcccttctaatgaaacaatac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: