XB-CLONE-3548617
Clone Name: IMAGE:7716105
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Gene:
anxa2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976514 [+]
agatcctgggcaagctgtccttggaaggaaatcaatcaagcacaagacagtcgacattaggttcagtcaaagcaagctcaaactttgatgcagaaaggga
tgcagcagccatcgaaactgccattaagactaaaggtgtggatgagttgacaatcatcaacattctaacaaaccgcagtaatgaacagagacaagacata
gcatttgcctaccacaggaaaacaaagaaggacctgccatctgcattaaagggagctctctctggcaatctggaaacttttatgttggggctaattaaga
ctccacctcagtatgatgcttcagaactgaaggcttcaatgaaggggctgggcaccgatgaagatagcttaattgagatcatctgctcccggaccaataa
agagttgctgaatattcagaatgcatacagagaattattcaagacagaactggaaaaagacattgtgtccgacacctctggtgatttccgcaaattaatg
gtggctcttgctaagggaaaacgccaggaagaatgtaatgtggtagattatgagaagattgaccaagatgccagggagctgtatgaagctggagtgaaga
ggaaaggaacagatgtgaacaaatggatctcaataatgacagaaagaagcatcccccaccttcagaaagtatttgaaagatacaagagctacagcccata
cgacatggaagagagcattaagaaagaagtgaaaggagatctggagaatgcctttttgaatctagttcagtgcatccagaacaaacccctgtattttgca
gacagattgtatgattcaatgaagggcagaggc
3' EST: CX976513 [+]aaatatggagtagttttattggcaaggaaacgggaatgtagcagtacagacgtacaattggctggatcactttgcagaccacaggcaagcagtgaagggg
agaacagatccagttttgttggaaatttagtcatctcctccacagaggttaaggagggcacgctggtaatcaccttttgtgtcttgcccaatgaagtaat
gtaacgatttgccatatttcttcttaaactctgatctgattttcagcatgtccgattcacttcgtgaaatcataattcggatcaagattttgtctttggt
gcctctgcccttcattgaatcatacaatctgtctgcaaaatacaggggtttgttctggatgcactgaactagattcaaaaaggcattctccagatctcct
ttcacttctttcttaatgctctcttccatgtcgtatgggctgtagctcttgtatctttcaaatactttctgaaggtgggggatgcttctttctgtcatta
ttgagatccatttgttcacatctgttcctttcctcttcactccagcttcatacagctccctggcatcttggtcaatcttctcataatctaccacattaca
ttcttcctggcgttttcccttagcaagagccaccattaatttgcggaaatcaccagaggtgtcggacacaatgtctttttccagttctgtcttgaataat
tctctgtatgcattctgaatattcagcaactctttattggtccgggagcagatgatctcaattaagctatcttcatcggtgccca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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